FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l02n02_musca_female_2.352000000b075b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l02n02_musca_female_2.352000000b075b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363908
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG23900.17523176049997508Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21520.15778190317822025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT2253.6379788E-1227.4433331
CGCGGCC1302.9427037E-421.922191
CCGGGGG1100.002878314621.5900341
CGCCGTA5150.020.29081246-47
CGGGAAT4550.018.790451
CTCGGTG5900.018.11367436-37
CGTATCA5950.016.76679248-49
TCGCCGT6700.016.6591644-45
AGGACCC2606.6272296E-616.4416433
GATCTCG6300.016.20995734-35
GCGGAAT3552.98096E-816.0556891
GATCCTA2104.0060907E-415.832115
GCCGTAT6500.015.7111946-47
TAGATCT7500.015.51660632-33
CGTACTG7100.015.3855728
TCTCGGT6650.015.35652136-37
GGGGTCT2205.703526E-415.1130251
TCGTACT8350.014.7887367
GCGGGTT3253.3728102E-614.6147921
GACGGTC1950.00446322314.6147922
Produced by FastQC (version 0.11.2)