FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l02n02_musca_female_1.352000000b0731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l02n02_musca_female_1.352000000b0731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493605
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG26980.18063678147836945Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23640.15827477813745938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACT1201.7113629E-423.7495672
CGCCGTA5800.021.29246-47
GATCTCG5800.021.29164334-35
CTCGGTG5900.020.93111836-37
AGATCTC5900.020.93041832-33
GGGGTCT2603.2823482E-718.2688941
TCTCGGT7150.018.26828436-37
CGTATCA7000.017.98301748-49
CGGGCTC1952.2762053E-417.050971
TAGATCT7450.016.57576832-33
GGACTCC2304.272971E-516.5214353
GGGGCAT3753.2832759E-916.4663661
GCCGTAT7650.016.45339646-47
GCGGATC2606.626684E-616.4420052
TCGCCGT7650.016.14295444-45
GTCGCCG7850.016.03420344-45
GTAGATC7750.015.93412630-31
AGTGTAG8300.015.45048928-29
CCGTATC8200.015.351356548-49
GCTATAG4502.2901077E-914.7765188
Produced by FastQC (version 0.11.2)