FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l02n01_musca_female_3.351000000b0777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l02n01_musca_female_3.351000000b0777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548003
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39280.25374627826948654No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC21060.13604624797238765TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG1200.004694140519.8424361
CGGGGAT2752.7570422E-819.0487381
TCTCGTA6300.018.47144540-41
GCGGGAT3259.713403E-917.583451
GCGGAAC2806.9473936E-717.0078031
GCGGGGG2555.3759923E-616.807711
CCGGGTT2951.1985085E-616.1429981
ATGCCGT7700.016.0372546-47
CCGGGAT1800.00257477515.8739491
CCGCGGT1850.0030907715.4449241
GGATCGA2851.5576721E-515.0248514
TATGCCG8250.014.39240546-47
CCTTTCG8050.014.1849533
CTCGTAT8050.014.16066742-43
CGGGATC3103.4341305E-513.8171952
GGGAAAT11400.013.7852711
CGGGTCT3153.968882E-513.6062421
TCACGCC8600.013.25654230-31
GCCGTCT8600.013.25354548-49
GGGGATA4353.497662E-713.1289822
Produced by FastQC (version 0.11.2)