FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l02n01_musca_female_2.351000000b075e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l02n01_musca_female_2.351000000b075e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363908
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.2574220548600052No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC23910.17530507922821773TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG4700.022.23349438-39
CGGGACT1302.8996117E-421.9700641
CCGGGGG1302.9140533E-421.9539282
CGGGTTA1100.002854249421.6212942
CCGGGAT2151.0571621E-619.9263361
TCTCGTA5750.018.58717740-41
GGGGCTA2202.8828086E-517.2970352
GCGAGAC2053.2786836E-416.2542741
GCGGGGT2053.2786836E-416.2542741
ATGCCGT7350.015.83294246-47
GGGGGCT3051.7036764E-615.6071491
CGGGCCT2751.10544515E-515.5787731
TGATCTC7100.015.38694438-39
GTGATCT6900.015.14788636-37
GCGGGTT3303.8630915E-614.4247891
CTCGTAT7750.014.403398542-43
AGTGATC7350.014.22046536-37
GCGGGAT2701.6470315E-414.10423851
GGGAATC4051.4735633E-714.093882
CGTATGC8500.013.97075544-45
Produced by FastQC (version 0.11.2)