FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l02n01_musca_female_1.351000000b0734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l02n01_musca_female_1.351000000b0734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493605
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41390.2771147659521761No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC26840.17969945199701393TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT1452.2998634E-522.9759881
CGCGGCC1150.003681776320.692661
TCTCGTA6300.018.85074240-41
CTCGTAT7200.017.81454842-43
AATCTCG6800.017.81096638-39
CCGGGTT2453.6936017E-617.4831851
AGTCACT7150.017.27095228-29
CGGACGT1952.2420962E-417.0847071
ACCTAAC2253.5281497E-516.894796
CAGTCAC8000.016.03309226-27
CCGGGAC2103.944821E-415.8643711
GCCGGAT4208.385541E-1015.8643711
ATGCCGT8200.015.35134546-47
GCCGTCT7900.015.33070448-49
CGGGAAG2205.6180696E-415.1432641
CGGGGAG2551.01451435E-414.9311721
CGCGGAT2952.1514394E-514.5199341
GCGGATC3002.5323214E-514.2712322
TATGCCG9300.014.04637246-47
GCTATAG4402.7153874E-814.0314758
Produced by FastQC (version 0.11.2)