FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l01n02_musca_male_3.352000000b0781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l01n02_musca_male_3.352000000b0781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585845
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10040.17137638795244475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAT1257.3560004E-626.6124571
CCGGTCT1404.832545E-420.3666761
TAAGTCC1200.004761975319.7907949
CCTTTCG3003.6907295E-919.0056533
TCGTACT3208.250936E-917.8117167
CACCGTA1350.00939744217.5918189
CGTACTG3007.664676E-817.41598
TTCGTAC3803.8980943E-916.2492856
GGGGAAT3102.0485022E-615.3297561
CGGGCAT1900.00373932815.0070251
AGTGACT1900.00375209514.9993396
CGGAATC2256.73822E-414.7821742
GGGAAGG1950.00444918514.6197332
CGGGTTT3002.5515808E-514.2566741
GGCAGGT2000.00525754814.2566741
GCTATAG2000.005275449714.24937258
CACATGC2050.006213739513.9018279
GGGCTAT2450.001281466213.57778551
GTACTGA3507.247385E-613.570839
GGGACAT2150.00848652113.2620221
Produced by FastQC (version 0.11.2)