FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l01n02_musca_male_2.352000000b0767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l01n02_musca_male_2.352000000b0767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563364
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5670.10064540865231004Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG405.3797005E-447.5109631
CGGGGTC405.3797005E-447.5109631
GCCGGAT1153.8530397E-628.9197181
GCGCGTT908.9298177E-426.3949811
TCGAGGC1456.134834E-419.6579652
CGGCACT1350.00938214817.5966531
CGCCGTA1902.0736479E-1017.49937846-47
CCAGAAT2555.484095E-616.7685741
CGGGAAT2103.9915176E-415.83698751
GAATCGT2952.1838685E-514.4935834
GGCGGTT2000.00526550614.253291
CTGGGAT3354.5932284E-614.1823781
GTCGCCG2558.8766683E-1013.97009244-45
CGTACTG2050.006205693413.9044133
TTCGGTC2050.006205693413.9044132
GCCGAAT3103.4823082E-513.7935061
GCGAAAT2802.2751284E-413.5745611
GATCTCG2304.8514266E-813.42343634-35
GGAATCG3558.372857E-613.3821823
ATCTATC2150.00851428713.2553446
Produced by FastQC (version 0.11.2)