FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l01n02_musca_male_1.352000000b074e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l01n02_musca_male_1.352000000b074e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684361
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7360.1075455790145844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTG2005.38148E-721.3741938
CGGGGAT1250.006017303619.0062261
CCTTTCG2502.1663618E-719.0020623
GCGGATA1801.2313714E-418.4742262
CGCCGTA2350.018.19080446-47
GCGGGTT1600.001187732417.8183361
CTCGGTG2700.017.59192836-37
GGTCGCC2850.015.83273742-43
GATCTCG2850.015.83273734-35
GTACTGA2709.469337E-615.8327359
TCTCGGT3000.015.041136-37
GGGCTAT2000.005263652714.254671
GCCGTAT3003.6379788E-1214.24946346-47
CGACCTA1000.005279998814.24946252-53
AGATCTC3055.456968E-1214.01586632-33
CCGGAAT2450.00128321213.5758771
TCGTACT3204.704944E-513.3598477
GTCGCCG3401.8189894E-1213.27155944-45
ATCTCGG3251.4551915E-1113.15335134-35
CGTATCA3301.8189894E-1112.95405748-49
Produced by FastQC (version 0.11.2)