FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l01n01_musca_male_3.351000000b0784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l01n01_musca_male_3.351000000b0784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585845
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.2432383992352926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT950.001216251625.033681
ACTGAGT1050.002198478422.612796
CGCGGTA1200.00473785819.8081682
GTACTGA2302.0597963E-618.5818129
CGTACTG2101.957796E-518.090238
CGGCGAT2152.3636565E-517.6982291
CCTTTCG2253.5127916E-516.9000823
TCGTACT2858.589577E-716.6620547
GAATGCA3251.8929859E-716.087584
AGAATGC2406.104378E-515.8438283
GTCCTTT3806.739174E-815.0202081
GCCGGTT3002.530532E-514.26919651
GCTGTAC2000.005227003214.26919651
TCCTATA2000.005283602514.2460577
GGGATAT2359.291959E-414.1679971
ATCGAGT2150.00852840213.2521466
CGGCATC3609.604997E-613.2054452
AATGCTA4657.988601E-712.2609515
TTCGTAC3501.0914812E-412.2109056
CTGCATT3952.4792918E-512.0415171
Produced by FastQC (version 0.11.2)