FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l01n01_musca_male_2.351000000b076a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l01n01_musca_male_2.351000000b076a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563364
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.14537670138667005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTA1050.002199292222.6111647
GCGGGCT1302.9054316E-421.9602681
GGGGACA1150.003703616620.6688273
GCGGGAT1759.797023E-519.0322281
GGAACTC1250.005993253619.01872
GGGTTCA1759.864111E-519.0153184
GCTCGAC1250.00601482519.0068745
GGATAGA2202.892821E-517.2866544
GCCGTCT2351.4551915E-1116.17031948-49
ATGTAGC2951.2320579E-616.0960856
CGTACTG2709.491338E-615.8278168
CCTTTCG2751.1171931E-515.5579883
GTACTGA3102.065648E-615.3172419
GGGGTTT2801.3139987E-515.2937571
GGGGAAT2851.5535925E-515.0254461
GTCCCGT950.00374483515.00542746-47
TCTAAGA1900.003759463614.9947747
CTGGGAT2901.8312294E-514.7663851
TTCGTAC3555.211932E-714.71318156
GGGGTGA1950.004468039614.61029159
Produced by FastQC (version 0.11.2)