FastQCFastQC Report
Fri 22 Dec 2017
H7F7FBCX2_l01n01_musca_male_1.351000000b0741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7F7FBCX2_l01n01_musca_male_1.351000000b0741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684361
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.14612171061764187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAC650.00581083829.2632271
GATCTCG2050.023.16595638-39
GCGACAC1656.264207E-520.1738931
CCGGGAT1250.005990176519.02111
CCGGGAG1250.005990176519.02111
TCTCGTA2650.018.82650440-41
GGGAGAC1700.001773349616.7833211
CTCGAAT2304.2264655E-516.5400871
GGGGGAT2355.081E-516.188171
CTCGTAT3200.015.59069842-43
ATGCCGT3100.015.3233446-47
GCGGGAT1900.003724248815.0166571
GCGGGAG2851.5631938E-515.0166571
CGATCTC3100.014.55345238-39
GCCGTCT3550.014.046448-49
ACGATCT3650.013.66156836-37
CCGAGGC2100.007234690713.58651
CGGGAGG2100.00725924313.5795452
CGTATGC3401.8189894E-1213.276113544-45
CGGGATG2150.00848116313.2637422
Produced by FastQC (version 0.11.2)