FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n02_smallrna_salt24h_rep2_root.35200000074994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n02_smallrna_salt24h_rep2_root.35200000074994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25144930
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT406059016.148742509921483Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT2917661.1603372926470665Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA1574600.626209736913167No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1137290.4522939614467012Illumina RNA PCR Primer (100% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1130620.4496413392282262Illumina DpnII expression Sequencing Primer (96% over 30bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1079010.42911632683010054No Hit
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG877850.34911610412118865Illumina RNA PCR Primer (100% over 34bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA821630.3267577201447767No Hit
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC714360.2840970326821351Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC658490.26187784177565815No Hit
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC640240.25461991741476314Illumina RNA PCR Primer (100% over 45bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG603400.2399688525678934Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA562240.2235997475435406Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTA485450.19306078799980753No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG480360.19103652306846747Illumina RNA PCR Primer (100% over 34bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT459600.18278038554889595Illumina RNA PCR Primer (100% over 38bp)
GGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCG419250.1667334130578212No Hit
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG386300.15362937975965732Illumina RNA PCR Primer (100% over 33bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG379810.1510483425485774Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG376620.1497796971397415Illumina RNA PCR Primer (100% over 36bp)
CGGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG370030.14715889048010872Illumina RNA PCR Primer (100% over 37bp)
CGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT358530.14258540389653102Illumina RNA PCR Primer (100% over 31bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT356010.1415832137929992Illumina RNA PCR Primer (100% over 35bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT341200.13569335846232222No Hit
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT336060.13364920880670578Illumina RNA PCR Primer (100% over 35bp)
GACTACAATTCGGACGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG334510.1330327823541366Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT327150.13010575094064689Illumina RNA PCR Primer (100% over 38bp)
TCCAGACTACAATTCGGACGGCGGATCGTCGGACTGTAGAACTCTGAACGT325550.1294694397638013Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCCAGGAGCGCATCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTG322680.12832805659033453Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC319690.12713895007860432Illumina RNA PCR Primer (100% over 39bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT315770.1255799876953326Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC314480.12506696180900087Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG307300.12221151540290627Illumina RNA PCR Primer (100% over 37bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT306640.12194903704245746Illumina RNA PCR Primer (97% over 46bp)
TCGGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG304560.1211218325125582Illumina RNA PCR Primer (100% over 36bp)
GGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCAC299820.11923676065115313No Hit
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG286490.11393549315905831No Hit
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT284810.11326736642337043Illumina RNA PCR Primer (100% over 38bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC261500.10399710796570123Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC258990.10299889480702472Illumina RNA PCR Primer (100% over 39bp)
GGAGCCCGCGTCAGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC258670.1028716325716556Illumina DpnII expression Sequencing Primer (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4353600.043.6323745
ATAACCT110100.042.77995310
GGGAATG330850.042.725732
GATAACC110500.042.522469
ACTATAC184600.042.00731318
CCGTATC131800.041.9992945
CACTATA184900.041.92698717
CGATAAC112850.041.8552978
CGCCGTA4625200.041.84802244
ACGATAA114150.041.654577
GGTCGCC4648150.041.65422441
TAACCTA111250.041.6295811
GGAATAC187300.041.6205566
TATACTA187150.041.57985720
TCGCCGT4653800.041.5540243
TGGTCGC4679650.041.3585740
AACGACG188250.041.13240428
GGGAATA189200.041.099715
CTATACT190250.040.9132619
GTCGCCG4736250.040.9003142
Produced by FastQC (version 0.11.2)