FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n02_smallrna_salt24h_rep1_root.3520000007497a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n02_smallrna_salt24h_rep1_root.3520000007497a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18898890
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT446183823.60899502563378Illumina RNA PCR Primer (100% over 46bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA4777552.5279526998675586No Hit
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC1214510.6426356256901861Illumina RNA PCR Primer (100% over 45bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1172640.6204808853853322Illumina RNA PCR Primer (100% over 45bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA1107390.5859550481536216No Hit
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT846410.44786228185888166Illumina DpnII expression Sequencing Primer (96% over 30bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN818260.4329672271757759No Hit
ACAACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTA629610.3331465498767388No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG516980.27355045719616333Illumina RNA PCR Primer (100% over 34bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC478150.2530042769707639No Hit
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG461160.2440143310003921No Hit
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG412120.2180657170870882Illumina RNA PCR Primer (100% over 34bp)
CGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT395090.20905460585251304Illumina RNA PCR Primer (100% over 31bp)
GGCCGCCCGATTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT386670.20459931773770842Illumina RNA PCR Primer (100% over 31bp)
GACTACAATTCGGACGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG369050.19527601885613388Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG362720.19192661579595416Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT358690.1897942154274669Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG339430.17960314071355515Illumina RNA PCR Primer (100% over 33bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT334190.17683049110291663Illumina RNA PCR Primer (97% over 46bp)
GGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCAC298710.15805690175454748No Hit
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC284700.15064376796732507Illumina RNA PCR Primer (97% over 45bp)
GGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCG277270.14671232014155328No Hit
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC275410.14572813535609763Illumina RNA PCR Primer (97% over 47bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT272190.14402433158772818Illumina RNA PCR Primer (100% over 35bp)
GGAGCCCGCGTCAGCCGATCGCGGACTGTAGAACTCTGAACGTGTAGATCT258980.13703450308457268Illumina RNA PCR Primer (96% over 31bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC254400.13461108033328942Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGATTCTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT244420.12933034691455425Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGACAGGGTCCGGGCCGGACGGGGCGATCGTCGGACTGTAGAACTCTGA236480.12512904197018979Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG234820.12425068350575086Illumina RNA PCR Primer (100% over 37bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACGATCGTCGGACTGTAGAA230420.12192250444338266No Hit
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT230380.12190133917917931Illumina RNA PCR Primer (97% over 46bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA224250.11865776244001632Illumina RNA PCR Primer (100% over 40bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC221180.1170333284124094Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGAA216590.1146046143450753No Hit
AGCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG214650.11357809903121295Illumina RNA PCR Primer (100% over 34bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT200330.10600093444641458Illumina RNA PCR Primer (100% over 31bp)
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT198740.10515961519433152Illumina RNA PCR Primer (100% over 35bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG198130.10483684491523049Illumina RNA PCR Primer (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA525200.043.66060617
ACTATAC525050.043.66022518
GGAATAC527450.043.630476
CTATACT527600.043.58991619
TATACTA528050.043.5539320
GGGAATA527600.043.530795
TACTACA529100.043.4450822
ATACTAC530100.043.41898721
AACGACG530750.043.3131928
CCGTATC222950.043.3103745
AGGCGGG526150.043.2996251
GCGGGAA530900.043.2617343
ACAACGA532800.043.1885426
ATAACCT85750.043.12455410
CGATAAC86350.042.9787988
CAACGAC535550.042.97483427
GCCGTAT4877850.042.91760645
ATACTCA535100.042.8376969
AATACTC538000.042.7937778
GAATACT539500.042.6581237
Produced by FastQC (version 0.11.2)