FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n02_smallrna_heat_1h_rep3_root.352000000749d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n02_smallrna_heat_1h_rep3_root.352000000749d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30538574
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1859019960.874482875330074Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5898091.9313573711726029Illumina RNA PCR Primer (100% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC3950201.2935116092847034Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA3141381.0286596879081518Illumina RNA PCR Primer (97% over 45bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT1497450.4903470607370206Illumina RNA PCR Primer (97% over 46bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1381810.4524801976673829No Hit
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC955050.31273562413228595Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA929410.30433968527803557Illumina RNA PCR Primer (100% over 40bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC925810.3031608483094201Illumina RNA PCR Primer (97% over 47bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT744040.24363940503574266Illumina RNA PCR Primer (97% over 46bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT344040.11265751963402089Illumina RNA PCR Primer (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC718900.043.96318445
GCCGTAT20081650.043.39729745
ATAACCT42900.042.4695310
AGCCTGA217150.041.9893231
ACGATAA44200.041.930397
CGATAAC44500.041.7987868
CGCCGTA21400650.041.5741144
TCGCCGT21395900.041.5373843
GGTCGCC21456400.041.48050341
TGGTCGC21458600.041.4571640
GTCGCCG21486350.041.44799442
GTGGTCG21487950.041.399939
GGAATAC35950.041.3532876
GATAACC44900.041.3277979
GGTGGTC21558450.041.27713838
CCACGGG109900.041.257411
TAACCTA42950.041.21522511
GCCGTAA411000.041.20579545
CGGTGGT21642450.041.12137637
ACGATGT115900.041.0247047
Produced by FastQC (version 0.11.2)