FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n02_smallrna_heat_1h_rep2_root.352000000749bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n02_smallrna_heat_1h_rep2_root.352000000749bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14278010
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT632893644.32645725839946Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1669541.1693086081323658Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA1269240.888947409337856Illumina RNA PCR Primer (97% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC1008420.7062748940503614Illumina RNA PCR Primer (100% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT1007600.7057005843251265Illumina DpnII expression Sequencing Primer (96% over 30bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN628430.44013836662111877No Hit
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT423040.29628778800407063Illumina RNA PCR Primer (97% over 46bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC310150.21722214790436484Illumina RNA PCR Primer (97% over 47bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC299230.20957402327074992Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA254400.17817609036553414Illumina RNA PCR Primer (100% over 40bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT210460.14740149362551222Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT202150.14158135482465695Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT196550.13765923962793133Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG194270.13606237844069308Illumina RNA PCR Primer (100% over 34bp)
AGGTGGGTTTCCCCATTCAGAAATCTCCGGATCAAAGCTTATTTGCCAGCT193810.13574020469239062No Hit
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT178920.12531158053538274Illumina RNA PCR Primer (97% over 46bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG147670.10342477698222652Illumina RNA PCR Primer (100% over 34bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT142990.10014700928210583Illumina RNA PCR Primer (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC198150.043.75385345
GCCGTAT6780950.043.695945
GGGAATG112750.043.37182
ATAACCT25650.042.9700110
GGGATGC11200.042.4549832
CGATAAC26650.042.283238
GCCGTAA154100.042.22863445
ACGATAA26800.042.1304977
CGCCGTA7214500.042.03613744
TCGCCGT7222550.041.9603143
GGTCGCC7232000.041.9445841
GATAACC26750.041.8748329
GTCGCCG7249900.041.86646742
TGGTCGC7248000.041.84359740
AGCCTGA102250.041.786171
GTGGTCG7273250.041.69557639
CCTGACG104350.041.4389423
GTGACGC21350.041.4351043
GGTGGTC7333200.041.36113438
GCGTAAT603.6379788E-1241.25274345
Produced by FastQC (version 0.11.2)