FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n02_smallrna_drought_1h_rep3_root.35200000074a36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n02_smallrna_drought_1h_rep3_root.35200000074a36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17373920
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT8369414.817226049158739Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT2444861.4072011382578025Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG1186010.6826381150598138No Hit
AGGTGGGTTTCCCCATTCGGAGATCTATGGATCAAAGCTTATGATCGTCGG747950.43050157937874706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN736460.4238882186633759No Hit
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT707140.4070123495446048Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG676540.3893997439840865No Hit
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG564610.3249755956053671Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT564190.3247338539604188Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT473980.2728112020775968Illumina RNA PCR Primer (100% over 35bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC445780.25657997734535437Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG409210.2355311869744997Illumina RNA PCR Primer (100% over 36bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT402840.23186477202611733Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG392650.2259996592593957Illumina RNA PCR Primer (100% over 34bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA379110.2182063690865389No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG356580.2052386565610985Illumina RNA PCR Primer (100% over 34bp)
AGTCCGTGGGTCTACCGTTAACCTATTCCCCAGATCGTCGGACTGTAGAAC352230.2027349038098483No Hit
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC349750.20130747695396317No Hit
TGTCAGATTAAAGTATTCTCAGCACCCTTGATCGTCGGACTGTAGAACTCT339890.19563230405112952Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG335140.19289832116183336Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGATTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT330020.1899513753948447Illumina RNA PCR Primer (100% over 31bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT271650.1563550425004835Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA263840.15185979905513552Illumina RNA PCR Primer (100% over 45bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC252420.14528672861392247Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG245790.14147066407581016Illumina RNA PCR Primer (100% over 37bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC236340.13603147706447363Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG230460.1326470940351976Illumina RNA PCR Primer (100% over 34bp)
GTGTCAGATTAAAGTATTCTCAGCACCCTTGATCGTCGGACTGTAGAACTC225390.12972892703546465Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA221020.1272136627773122Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT220860.12712157072209382Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC215880.12425520550342123No Hit
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG214920.12370265317211084Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCAGGTGCTCTCCCAGCTGAGCTACAGCCCCGATCGTCGGACTGTAGAA211670.12183203330048717No Hit
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT208560.12004199397717959Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG205520.11829224492803006Illumina RNA PCR Primer (100% over 34bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT201270.11584604971129141Illumina RNA PCR Primer (100% over 35bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA195110.11230050558538315Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA190820.1098312873548399Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG189970.10934204831149216Illumina RNA PCR Primer (100% over 40bp)
GCCGTTACTAGGGGAATCCTCGTAAGTTTCTTCTCCTCCGCTTAGATCGTC189910.10930751379078527No Hit
AGCCTGACGATGTCCTACTTGATCGTCGGACTGTAGAACTCTGAACGTGTA189200.10889885529575362Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGAATCCTCGTAAGTTTCTTCTCCTCCGCTGATCGTCGGACTGTAGAAC187880.10813909584020186No Hit
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT176960.10185381307154633Illumina RNA PCR Primer (100% over 35bp)
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG173860.10006952950168989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT886200.043.70059245
ATAACCT76950.042.7058410
CGATAAC77550.042.604878
GATAACC77700.042.494789
AGCCTGA368200.042.171441
GGGAATG294500.041.1285552
CGCCGTA967300.041.03652244
TGGCCTC300400.040.9042781
ACGATGT214350.040.827667
ACGATAA81550.040.7079437
CCTGACG388050.040.697333
GGAATAC46350.040.565986
TCGCCGT979950.040.48830443
CACACTG61250.040.47439222
TATACTA46550.040.30544720
ACACTGG58000.040.3010423
GGTCGCC990700.040.08723441
ACGAATC253350.039.9307331
CGATGTC220050.039.7805448
AAATCTA45600.039.7370033
Produced by FastQC (version 0.11.2)