FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n02_smallrna_drought_1h_rep2_root.35200000074a1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n02_smallrna_drought_1h_rep2_root.35200000074a1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22482141
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT582114925.892324934711514Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT2739311.2184382261458107Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1567720.697317928928566Illumina RNA PCR Primer (100% over 45bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG1374290.6112807494624289No Hit
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT1036120.4608635805637906Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA1034740.4602497600206315Illumina RNA PCR Primer (97% over 45bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN963670.4286380020479367No Hit
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT824860.36689566176103955Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG673610.29962004063580955No Hit
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC660050.2935885866030286Illumina RNA PCR Primer (100% over 45bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG499560.2222030366236027Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG473880.2107806369509025Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG461110.2051005729392054Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG437560.19462559193094642Illumina RNA PCR Primer (100% over 37bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT432840.19252614775434423Illumina RNA PCR Primer (97% over 46bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT393480.1750189183494579Illumina RNA PCR Primer (100% over 38bp)
TGTCAGATTAAAGTATTCTCAGCACCCTTGATCGTCGGACTGTAGAACTCT336960.14987896392963643Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA308940.13741573811853594Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC307660.1368463973248811Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG280170.12461891418615335Illumina RNA PCR Primer (100% over 34bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC265960.11829834178159455No Hit
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT257780.11465989827214411Illumina RNA PCR Primer (100% over 38bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA252110.11213789647525117Illumina RNA PCR Primer (100% over 40bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC238110.10591073154465139Illumina RNA PCR Primer (97% over 47bp)
AGCCTGACGATGTCCTACTTTCACACGGGATCGTCGGACTGTAGAACTCTG225180.10015949993374741Illumina DpnII expression Sequencing Primer (100% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT6164150.043.94332545
TGGCCTC294900.043.2257041
CCGTATC148500.042.42886445
CGCCGTA6537250.042.30687744
TCGCCGT6540700.042.24912643
ATAACCT52650.042.20857610
GGTCGCC6561200.042.1541941
TGGTCGC6581550.042.0188740
GTCGCCG6602100.041.9248342
GATAACC53750.041.846389
CGATAAC54150.041.7434548
GTGGTCG6643650.041.632239
GGGAATG319200.041.3005942
TAACCTA53850.040.7248211
GGTGGTC6799600.040.69402738
ACGATGT134400.040.548977
ACGATAA56600.040.334377
GGCCTCG320550.040.2689742
AGCCTGA236600.040.202761
CCTGACG241000.040.153573
Produced by FastQC (version 0.11.2)