H72FYBCXY_l02n02_smallrna_drought_1h_rep1_root.352000000749f1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l02n02_smallrna_drought_1h_rep1_root.352000000749f1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12781849
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	5673854	44.38993137847271	Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	124837	0.976674032059055	Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	96979	0.7587243441852584	Illumina RNA PCR Primer (97% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC	76742	0.6003982678875334	Illumina RNA PCR Primer (100% over 45bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	56937	0.44545198429429106	No Hit
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	47890	0.374671927355737	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	36808	0.28797085617268675	Illumina RNA PCR Primer (97% over 46bp)
GGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTACC	28872	0.22588281241626307	No Hit
TTCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGATCG	28476	0.22278466910382058	No Hit
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	26546	0.207685132252775	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC	25990	0.20333521386459813	Illumina RNA PCR Primer (97% over 47bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	24906	0.19485443772649794	Illumina RNA PCR Primer (100% over 40bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC	22253	0.1740984422519778	Illumina RNA PCR Primer (97% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	20046	0.1568317698010671	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	19106	0.14947759123112783	Illumina RNA PCR Primer (100% over 34bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	14924	0.11675932018912132	Illumina RNA PCR Primer (100% over 32bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	14397	0.11263628603342131	Illumina RNA PCR Primer (97% over 46bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	603340	43.80094	45
CCGTATC	16225	43.202274	45
CGCCGTA	636250	42.375153	44
TCGCCGT	636515	42.321285	43
GGTCGCC	637840	42.270267	41
TGGTCGC	638855	42.192074	40
GTCGCCG	639985	42.156723	42
GTGGTCG	641090	42.045578	39
GGTGGTC	645830	41.748768	38
CGGTGGT	651665	41.381817	37
ACGATGT	6180	41.271717	7
GCCGTAA	12070	41.201996	45
TCGGTGG	657140	41.027798	36
CTCGGTG	662255	40.70529	35
ACGTGGG	1960	40.509335	9
CCACGGG	6060	40.37255	1
TTCACAC	8460	40.312103	20
ATAACCT	1660	40.243603	10
TCTCGGT	669900	40.241394	34
ACTTTCA	8935	39.93021	17

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content