FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n01_smallrna_salt24h_rep2_root.35100000074997.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n01_smallrna_salt24h_rep2_root.35100000074997.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25144930
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC396604015.772722373854291RNA PCR Primer, Index 9 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2759401.0973981633673269RNA PCR Primer, Index 1 (100% over 30bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA1420180.564797754457857No Hit
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1382210.54969729484234RNA PCR Primer, Index 6 (100% over 34bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG897220.3568194463058756RNA PCR Primer, Index 9 (100% over 39bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC755330.30039057575423755No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA670410.2666183600431578RNA PCR Primer, Index 7 (97% over 35bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC631480.2511361137215335RNA PCR Primer, Index 1 (100% over 33bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA607750.24169882357994235No Hit
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG532220.2116609590879752RNA PCR Primer, Index 9 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACCAGATCTCGTATGCC498290.1981671851939934RNA PCR Primer, Index 9 (98% over 51bp)
CGCCGTCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCC463480.1843234401527465No Hit
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG457280.18185773434246985RNA PCR Primer, Index 9 (100% over 39bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTTGGAATTCTCGGGTG450610.1792051121239948No Hit
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA425660.16928263471005883RNA PCR Primer, Index 9 (100% over 38bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT398840.15861646860818462RNA PCR Primer, Index 9 (100% over 43bp)
GGCTGACGCGGGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA391560.1557212527535372RNA PCR Primer, Index 7 (97% over 35bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA380740.1514181984201189RNA PCR Primer, Index 9 (100% over 40bp)
GCCCCCGACGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT362270.14407278127240758RNA PCR Primer, Index 9 (100% over 36bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA355290.1412968737634187RNA PCR Primer, Index 7 (97% over 35bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT349670.13906183075474857RNA PCR Primer, Index 9 (100% over 41bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG345550.13742332947437116RNA PCR Primer, Index 9 (100% over 39bp)
CAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTC344860.13714892027935652No Hit
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA343060.1364330702054052RNA PCR Primer, Index 9 (100% over 40bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG321270.12776730736573932No Hit
CTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC318890.12682079449018152RNA PCR Primer, Index 9 (100% over 42bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC315550.12549249490851636RNA PCR Primer, Index 9 (100% over 42bp)
CGCCGTCCGAATTGTAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA311340.12381820112444138RNA PCR Primer, Index 1 (100% over 32bp)
CGCCGTCCGAATTGTAGTCTGGATGGAATTCTCGGGTGCCAAGGAACTCCA305350.1214360111561257RNA PCR Primer, Index 1 (100% over 28bp)
GCCGGCGATGCGCTCCTGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG303820.12082753859326711RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT301550.11992477211111743RNA PCR Primer, Index 9 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCACATCTCGTATGCC292450.11630575229280814RNA PCR Primer, Index 9 (98% over 51bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT287900.1144962423836535RNA PCR Primer, Index 1 (100% over 30bp)
GCGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC269830.10730990303015359RNA PCR Primer, Index 9 (100% over 44bp)
GGCGAGCACCGACCTGCTCGACCCTTCTGCCGGCGATGCGCTCCTGGCCTT266880.10613670429784454No Hit
CTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT260030.10341249707197435RNA PCR Primer, Index 9 (100% over 41bp)
CAACTCTTAGCAACGGATATCTAGGCTCTCGTATCGATGAAGAACGCAGCG254750.10131267018838391No Hit
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT253550.10083543680574972RNA PCR Primer, Index 9 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCC328000.043.9725814
GTATGCC4679300.043.94190645
TAGTATA189050.043.7739148
GTATAGT188350.043.76940510
TATAGTG190000.043.40114611
CGTATGC4739450.043.38794344
TCGTATG4740050.043.37580543
CGTTGTA270950.043.2927363
TCGTTGT167300.043.2449072
ATCTCGT4751400.043.12478640
AGTATAG191900.043.006599
CTCGTAT4780300.042.95223642
TCTCGTA4772450.042.94558741
GTATTCC190750.042.7605922
TATTCCC190700.042.73641223
TGTAGTA191600.042.670726
GATCTCG4677400.042.6218139
GTAGTAT195000.042.3805777
TTCCCGC192650.042.2808225
TAGTCTA122250.042.209178
Produced by FastQC (version 0.11.2)