FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n01_smallrna_salt24h_rep1_root.3510000007497d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n01_smallrna_salt24h_rep1_root.3510000007497d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18898890
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC445934623.595809066035095RNA PCR Primer, Index 2 (100% over 51bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA4288382.269117392608772No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC1021380.540444438800374No Hit
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT801490.42409369015852255RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGCC614420.3251090407955176RNA PCR Primer, Index 2 (98% over 51bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTTGGAATTCTCGGGTG588040.31115054905341No Hit
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT505430.2674389871574468RNA PCR Primer, Index 2 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGCATCTCGTATGCC502480.2658780489224499RNA PCR Primer, Index 2 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC486530.2574383998213652RNA PCR Primer, Index 2 (100% over 34bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA440960.23332587257770165No Hit
GGCGAGCACCGACCTGCTCGACCCTTCTGCCGGCGATGCGCTCCTGGCCTT439370.2324845533256186No Hit
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT431380.22825679180099998RNA PCR Primer, Index 2 (100% over 39bp)
GCCCCCGACGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA411000.21747308968939447RNA PCR Primer, Index 2 (100% over 36bp)
GGCTGACGCGGGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG383350.2028426008088306RNA PCR Primer, Index 2 (100% over 35bp)
GAGAATCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA381390.20180550286286653RNA PCR Primer, Index 2 (100% over 36bp)
CAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTC347070.18364570617639447No Hit
CGCCGTCCGAATTGTAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA345970.18306366141080244RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG336930.17828031170084593RNA PCR Primer, Index 2 (100% over 35bp)
GCCGGCGGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG326830.1729360824895007RNA PCR Primer, Index 2 (100% over 38bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT324140.1715127184718256RNA PCR Primer, Index 2 (100% over 39bp)
CGCCGTCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCC297230.15727378697902364No Hit
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA282720.14959608738925936RNA PCR Primer, Index 2 (100% over 40bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG280200.14826267574444849RNA PCR Primer, Index 2 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGACGTATCTCGTATGCC278600.1474160651763146RNA PCR Primer, Index 2 (98% over 51bp)
CAACTGTTAACAATGGATATCTTGGCTCTTATAACGATGAAGAACGCAGCA263180.13925685582592418No Hit
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG233920.12377446506117555RNA PCR Primer, Index 2 (100% over 35bp)
GCCCCGTCCGGCCCGGACCCTGTCGCCTGGAATTCTCGGGTGCCAAGGAAC226760.11998588276877636RNA PCR Primer, Index 1 (100% over 24bp)
TTGAGAATCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC224240.11865247112396549RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA223160.11808100899047509RNA PCR Primer, Index 2 (100% over 36bp)
GTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCCA221400.11714973736552782No Hit
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC218050.11537714648849748RNA PCR Primer, Index 1 (100% over 33bp)
GGGCGGCGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT217170.11491151067602383RNA PCR Primer, Index 2 (100% over 39bp)
CTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC205930.10896407143488321RNA PCR Primer, Index 2 (100% over 42bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG200620.10615438261188884RNA PCR Primer, Index 2 (100% over 38bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGGAATTCTCGGGTGCCA198180.10486330149548466No Hit
CCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA194290.10280497955170913RNA PCR Primer, Index 2 (100% over 40bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT193260.10225997399847292RNA PCR Primer, Index 2 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATA533350.044.6505748
GTATAGT531250.044.6026710
AGTATAG531300.044.5942349
TCGTTGT467550.044.5924072
TGTAGTA528600.044.570316
TATAGTG531650.044.54795511
CGTTGTA500250.044.480483
TATTCCC532800.044.44922623
GTATTCC532800.044.4323422
GTAGTAT535600.044.4252057
GTTGTAG510750.044.3072974
TTCCCGC534800.044.26231825
TCCCGCC537500.044.1320526
GTATGCC5260200.043.8788245
GAGTATT540850.043.59066420
TGAGTAT541750.043.5390119
GTCGTTG479700.043.466371
AGTATTC544900.043.45656621
CCCGCCT540700.043.37162827
CGTATGC5340950.043.21523344
Produced by FastQC (version 0.11.2)