H72FYBCXY_l02n01_smallrna_heat_1h_rep3_root.351000000749da.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l02n01_smallrna_heat_1h_rep3_root.351000000749da.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30538574
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	19266359	63.088600666160765	RNA PCR Primer, Index 11 (100% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	97389	0.31890487093470704	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCTACATCTCGTATGCC	78293	0.2563741188439251	RNA PCR Primer, Index 11 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	74118	0.24270288455512037	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCCACATCTCGTATGCC	72920	0.2387799770873388	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCTACATCTCGTATGCC	72101	0.23609812298373856	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACTACATCTCGTATGCC	59010	0.19323102643889004	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGATACATCTCGTATGCC	56394	0.18466481113361743	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTACATCTCGTATGCC	55450	0.1815736386381368	RNA PCR Primer, Index 11 (98% over 51bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	41756	0.13673199017085735	RNA PCR Primer, Index 11 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	41074	0.134498749024758	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	40523	0.1326944735533493	RNA PCR Primer, Index 11 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTCCATCTCGTATGCC	40274	0.13187911131672359	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTACATCTCGTATGCC	37874	0.12402019819262025	RNA PCR Primer, Index 11 (98% over 51bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	37074	0.12140056048458583	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGCTACATCTCGTATGCCG	36464	0.11940308673220956	TruSeq Adapter, Index 11 (96% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTGCATCTCGTATGCC	35254	0.11544088469880748	RNA PCR Primer, Index 11 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	35116	0.11498899719417154	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAGATCTCGTATGCC	34222	0.11206155205544306	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAATCTCGTATGCCG	33140	0.10851849205532647	RNA PCR Primer, Index 11 (97% over 38bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACGGCTACATCTCGTATGCCG	31334	0.10260465992943874	Illumina PCR Primer Index 11 (96% over 32bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	30872	0.10109181915304886	RNA PCR Primer, Index 11 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CTATATC	4315	44.57113	36
GCTATAT	4130	44.334953	35
ACGACTA	7070	44.097603	32
GTATGCC	2181185	44.07769	45
AGTTCTC	14135	43.92876	4
TCACAGC	7050	43.87223	30
CATCTCG	2165780	43.871662	39
TGCATCT	9045	43.844193	37
TCTCGTA	2189205	43.835567	41
ATCTCGT	2189045	43.834965	40
TCTACAT	10975	43.820274	35
CGTATGC	2195540	43.78596	44
TCGTATG	2195285	43.77667	43
CTCGTAT	2194460	43.776005	42
ATACATC	8880	43.77227	36
ACATCTC	2150945	43.766388	38
ACGTCTA	9195	43.71636	32
GTTCTCG	15550	43.697716	5
TACATCT	2137795	43.632397	37
TCACTGC	10815	43.595425	30

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content