H72FYBCXY_l02n01_smallrna_heat_1h_rep2_root.351000000749b0.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l02n01_smallrna_heat_1h_rep2_root.351000000749b0.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14278010
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC	6312341	44.210229576810775	RNA PCR Primer, Index 10 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	95678	0.6701073889148418	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTCATCTCGTATGCC	60188	0.4215433383223573	RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCATATCTCGTATGCC	48435	0.33922794563107883	RNA PCR Primer, Index 10 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC	34896	0.2444038069731006	RNA PCR Primer, Index 10 (98% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC	33399	0.23391915259899665	RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCGTATCTCGTATGCC	30183	0.21139500532637254	RNA PCR Primer, Index 10 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	26280	0.18405926316062252	RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACCTTATCTCGTATGCC	23968	0.16786653041985544	RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCCTATCTCGTATGCC	21563	0.1510224464053464	RNA PCR Primer, Index 10 (98% over 51bp)
GGGAGCTGGCAAATAAGCTTTGATCCGGAGATTTCTGAATGGGGAAACCCA	21240	0.14876022639009218	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTGATCTCGTATGCC	19419	0.13600634822359697	RNA PCR Primer, Index 10 (98% over 51bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18561	0.12999710744004242	RNA PCR Primer, Index 1 (100% over 30bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC	18502	0.12958388458895884	RNA PCR Primer, Index 10 (98% over 51bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT	18458	0.12927571839493038	RNA PCR Primer, Index 10 (100% over 39bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT	18402	0.12888350687525782	RNA PCR Primer, Index 10 (100% over 43bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT	15111	0.10583407631735794	RNA PCR Primer, Index 10 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGCTTATCTCGTATGCC	15051	0.10541384968913736	RNA PCR Primer, Index 10 (98% over 51bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA	14718	0.10308159190251304	RNA PCR Primer, Index 10 (100% over 40bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT	14350	0.10050420191609336	RNA PCR Primer, Index 10 (100% over 39bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	728345	44.394726	45
CGTATCT	5610	44.14798	37
CGTATGC	734895	43.996433	44
TCTCGTA	734330	43.966568	41
TCGTATG	735640	43.951263	43
ATCTCGT	734845	43.938057	40
CTCGTAT	735755	43.932167	42
CATTCCC	11280	43.83108	14
TCACTAC	5720	43.613594	30
TTATCTC	704965	43.525883	38
CTAGCTC	6595	43.48893	33
CATCTCG	14135	43.382534	39
TATCTCG	725780	43.330147	39
CATATCT	7530	43.317104	37
GTCACTT	3285	43.072655	29
GCATATC	6200	43.06708	36
CTTATCT	711540	42.97651	37
GCGTATC	4440	42.96346	36
CACTACC	5295	42.865868	31
CTAGCAT	5305	42.657555	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content