FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n01_smallrna_drought_1h_rep3_root.35100000074a39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n01_smallrna_drought_1h_rep3_root.35100000074a39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17373920
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC7877924.53433652278818RNA PCR Primer, Index 7 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2288311.3170948179800528RNA PCR Primer, Index 1 (100% over 30bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT1202250.691985458664481No Hit
ATAAGCTTTGATCCATAGATCTCCGAATGGGGAAACCCACCTTGGAATTCT790780.45515347141002144No Hit
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC673990.38793202685404327RNA PCR Primer, Index 2 (100% over 34bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC657200.37826811680956285No Hit
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT639830.36827037306491567RNA PCR Primer, Index 1 (100% over 30bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC497970.28661925460690507RNA PCR Primer, Index 7 (100% over 39bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA464870.2675677106835993RNA PCR Primer, Index 7 (100% over 40bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA399440.22990781585272638RNA PCR Primer, Index 7 (100% over 35bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC393770.22664430364592447RNA PCR Primer, Index 7 (100% over 39bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT377220.21711853168427162RNA PCR Primer, Index 7 (100% over 41bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT363810.20940006630627978RNA PCR Primer, Index 7 (100% over 43bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA340660.19607549706686803No Hit
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC334440.1924954184202529RNA PCR Primer, Index 7 (100% over 39bp)
CCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATT328930.18932399826866936No Hit
TGGGGAATAGGTTAACGGTAGACCCACGGACTTGGAATTCTCGGGTGCCAA327320.18839732196303424No Hit
AAGGGTGCTGAGAATACTTTAATCTGACATGGAATTCTCGGGTGCCAAGGA324820.18695838360024683RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA321560.18508200797517196RNA PCR Primer, Index 1 (100% over 32bp)
GAGAATCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG320440.1844373635886432RNA PCR Primer, Index 7 (100% over 36bp)
AGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA253870.1461213128643392RNA PCR Primer, Index 1 (100% over 28bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC244430.14068788160645382RNA PCR Primer, Index 7 (100% over 42bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC243500.1401525965354969RNA PCR Primer, Index 1 (100% over 33bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC239660.13794238721025537RNA PCR Primer, Index 7 (100% over 39bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA233600.1344544006188586RNA PCR Primer, Index 7 (100% over 35bp)
CCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTC219460.12631576523893284RNA PCR Primer, Index 1 (100% over 26bp)
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATT215310.1239271275567057No Hit
AAGGGTGCTGAGAATACTTTAATCTGACACTGGAATTCTCGGGTGCCAAGG213570.12292562645620563Illumina Small RNA Adapter 2 (100% over 21bp)
CAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCC209540.12060605781539227RNA PCR Primer, Index 1 (100% over 27bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC208710.12012833027894684RNA PCR Primer, Index 7 (100% over 39bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA208250.11986356562019396RNA PCR Primer, Index 7 (100% over 40bp)
GGGGCTGTAGCTCAGCTGGGAGAGCACCTGCTTTGGAATTCTCGGGTGCCA203770.11728498807407886No Hit
TAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCTGGAATT197590.11372793244126829No Hit
CCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA195720.11265160654590328RNA PCR Primer, Index 7 (100% over 40bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT190000.10935931557184563RNA PCR Primer, Index 7 (100% over 43bp)
ATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAAC179940.10356902759998896RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACT179740.10345391253096595RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC953500.043.82885745
CATTCCT79400.043.6001816
CATTCCC282450.043.55484814
CACACGA96000.043.2134631
GTATAGT48250.042.28439710
CGTATGC991250.042.17721644
TAGTATA47500.042.0522778
CGTTGTA102650.041.7117043
GGTTAAC49150.041.1897510
TCGTATG1015200.041.17333243
CTCGTAT1015850.041.1159942
GACCCAC48950.040.89898321
GCTCAGC96400.040.81153510
AGCGCAG62350.040.80349
GTATTCC47400.040.7658322
TCTCGTA1023200.040.7480941
ATCTCGT1031050.040.44486240
GTAGTAT48750.040.3741727
AGACCCA49800.040.2460820
GCGCAGT33150.040.17060510
Produced by FastQC (version 0.11.2)