FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l02n01_smallrna_drought_1h_rep2_root.35100000074a1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l02n01_smallrna_drought_1h_rep2_root.35100000074a1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22482141
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC575152925.582656918662682RNA PCR Primer, Index 3 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2609491.1606946153393487RNA PCR Primer, Index 1 (100% over 30bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT1390450.6184686769823212No Hit
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1000370.44496207011600897RNA PCR Primer, Index 3 (100% over 34bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT949230.422215126219518RNA PCR Primer, Index 1 (100% over 30bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC658140.2927390233874968No Hit
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC494790.22008135257224837RNA PCR Primer, Index 3 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCGGCATCTCGTATGCC465900.2072311529404606RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTATGCATCTCGTATGCC449020.1997229712241374RNA PCR Primer, Index 3 (98% over 51bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC447030.19883782420900215RNA PCR Primer, Index 3 (100% over 42bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC444780.19783702984515575RNA PCR Primer, Index 3 (100% over 39bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT431560.19195680696068937RNA PCR Primer, Index 3 (100% over 41bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT362420.1612035081534272RNA PCR Primer, Index 3 (100% over 43bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC345320.1535974709881946RNA PCR Primer, Index 1 (100% over 33bp)
AAGGGTGCTGAGAATACTTTAATCTGACATGGAATTCTCGGGTGCCAAGGA328720.1462138325704834RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACGCATCTCGTATGCC320210.14242860588766879RNA PCR Primer, Index 3 (98% over 51bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT286130.1272699072566087RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGACATCTCGTATGCC276890.12315997840241283RNA PCR Primer, Index 3 (98% over 51bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC264830.11779572061219613RNA PCR Primer, Index 3 (100% over 39bp)
CCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATT252240.11219572014960674No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGGCATCTCGTATGCC241940.10761430595066547RNA PCR Primer, Index 3 (98% over 51bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT234730.10440731601140657RNA PCR Primer, Index 3 (100% over 43bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC6623700.044.49347345
TCTCGTA6669150.044.10521341
CGTATGC6683450.044.10070844
CTCGTAT6683000.044.07506642
CATTCCC306700.044.0714914
TCGTATG6690250.044.0471443
ATCTCGT6683750.044.0118840
CATTCCT120350.043.94159716
CATCTCG6662350.043.65377439
TACGCAT38150.043.16264735
GCATCTC6648450.042.84671838
TGCATCT87900.042.6869237
ATGCATC71750.042.60741836
TTACGCA37000.042.4370534
CACACGA63700.042.3290631
TATGCAT57900.042.27093535
CGTTGTA84450.041.919523
TCACTTC88600.041.91823230
AGCGCAG64400.041.7398959
TAATGCA17600.041.483194
Produced by FastQC (version 0.11.2)