H72FYBCXY_l02n01_smallrna_drought_1h_rep1_root.351000000749f4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l02n01_smallrna_drought_1h_rep1_root.351000000749f4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12781849
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	5564171	43.53181609327414	RNA PCR Primer, Index 1 (100% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	56244	0.44003023349751663	RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCCCGATCTCGTATGCC	47005	0.3677480464680814	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACCATCTCGTATGCC	42295	0.33089891767615154	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCACGATCTCGTATGCC	33256	0.2601814494913842	RNA PCR Primer, Index 1 (98% over 51bp)
AAGGCTCCGATCGGTAGCTGGTCTGAGAGGATGACCAGCCACACTGGGACT	31555	0.24687351571748345	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCTCGATCTCGTATGCC	29205	0.2284880692926352	RNA PCR Primer, Index 1 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	29073	0.22745535485515436	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAAGATCTCGTATGCC	28250	0.2210165368093458	RNA PCR Primer, Index 1 (98% over 51bp)
CTGAAGGGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAATGGAA	27913	0.21837998555608035	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAACGATCTCGTATGCC	23065	0.1804512007613296	RNA PCR Primer, Index 1 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	19065	0.1491568238679709	RNA PCR Primer, Index 1 (100% over 30bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG	18124	0.14179482170380828	RNA PCR Primer, Index 1 (100% over 39bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	15169	0.11867610077383954	RNA PCR Primer, Index 1 (98% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA	13774	0.10776218683228068	RNA PCR Primer, Index 1 (100% over 37bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT	13654	0.10682335552547992	RNA PCR Primer, Index 1 (100% over 43bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	644335	44.32026	45
TCACAGC	7160	44.141376	30
ATCTCGT	645885	44.0223	40
TCTCGTA	645955	44.01981	41
CGTATGC	648650	44.019672	44
TCGTATG	648895	43.996468	43
CTCGTAT	648080	43.957386	42
GATCTCG	633205	43.87362	39
CGATCTC	629105	43.607113	38
GTCACAG	7590	43.122486	29
ACGATCT	626335	42.86582	37
CTATCTC	2735	42.768784	38
ACCATCT	6460	42.691246	37
CACGATC	626305	42.60226	36
CCATCTC	8815	42.565193	38
TCACGAT	624825	42.428448	35
GCACGAT	4115	42.4207	35
CACAGCA	5620	42.34807	31
TCTACAT	760	42.326115	35
TAATGCA	980	42.312317	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content