FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l01n02_smallrna_heat_1h_rep1_root.35200000074a43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l01n02_smallrna_heat_1h_rep1_root.35200000074a43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11541825
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT564889248.9427971746236Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1700601.4734238302868046Illumina RNA PCR Primer (100% over 45bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC663860.5751776690428073Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA589260.5105431766640025Illumina RNA PCR Primer (97% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT469600.4068680646258282Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT402680.3488876325884338Illumina RNA PCR Primer (97% over 46bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT352630.3055236065353616Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG232070.20106872180092836Illumina RNA PCR Primer (100% over 34bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC219490.19016923233544086Illumina RNA PCR Primer (97% over 45bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN218690.18947610104987728No Hit
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA215770.18694617185757018Illumina RNA PCR Primer (100% over 40bp)
GATCGCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATC208450.18060402059466332Illumina RNA PCR Primer (97% over 47bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG189690.1643500919481971Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG185130.16039924362048463Illumina RNA PCR Primer (100% over 34bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA180120.15605850894464263No Hit
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT167480.14510703463273789Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT166130.14393737558834932Illumina RNA PCR Primer (100% over 38bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT151480.13124440892146608Illumina RNA PCR Primer (97% over 46bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC148100.12831592923995988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT6133550.043.92193645
CCGTATC148900.043.32204445
ATAACCT15350.042.49676510
CGCCGTA6485650.042.1225544
TCGCCGT6485200.042.06718443
GGTCGCC6505850.042.02116841
TGGTCGC6508600.041.9683140
GTCGCCG6520800.041.9243242
GTGGTCG6530900.041.84308639
GGGAATG59850.041.6923262
GATAACC15850.041.582659
GGTGGTC6580750.041.50697338
ACGATGT36550.041.171397
CGATAAC16150.041.0875328
CGGTGGT6654500.041.03557637
ACGAATC87500.040.797881
GGGAATA22450.040.687865
TCGGTGG6732100.040.5558836
ACGATAA16500.040.4882777
GCGGGAA22650.040.3236663
Produced by FastQC (version 0.11.2)