FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l01n02_smallrna_ctrl1h_rep3_root.35200000074a29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l01n02_smallrna_ctrl1h_rep3_root.35200000074a29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13678540
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT854764862.489476216028905Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1986701.4524210917247016Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA763470.5581516740821755Illumina RNA PCR Primer (97% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT488230.35693136840627726Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT471780.3449052311138469Illumina RNA PCR Primer (97% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT403710.2951411481049878Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT288990.2112725480935831Illumina RNA PCR Primer (97% over 46bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN267600.1956349142525445No Hit
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA232960.17031057408173678Illumina RNA PCR Primer (100% over 40bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA205880.15051313956021622Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG167420.12239610367773168Illumina RNA PCR Primer (100% over 34bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT160890.11762220236955115Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG159280.11644517616646222Illumina RNA PCR Primer (100% over 34bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA159220.1164013118359123No Hit
GATCATCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT155210.11346971241082747Illumina RNA PCR Primer (100% over 46bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC152410.1114227103184989Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT150210.10981435153166932Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT145090.10607126199141137Illumina RNA PCR Primer (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT9146800.044.5438245
CGCCGTA9570050.043.07831644
TCGCCGT9560000.043.0518143
GGTCGCC9592000.043.00061441
TGGTCGC9593150.042.97379340
GTGGTCG9606700.042.91996839
GTCGCCG9622300.042.86138542
GGTGGTC9649350.042.71005638
GGAATAC21150.042.540656
CGGTGGT9718950.042.3763337
GCCGTAA100450.042.15588445
TCGGTGG9807800.042.01170736
CTCGGTG9889150.041.65011635
GGGAATG51250.041.5539632
GCGGGAA21800.041.2789083
TCTCGGT10001950.041.18146534
GGGAATA21800.041.0768935
CGACGTA11450.041.07466544
TATACTA22500.040.9910820
CCGTATC95950.040.9906745
Produced by FastQC (version 0.11.2)