H72FYBCXY_l01n02_smallrna_ctrl1h_rep2_root.35200000074a0f.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n02_smallrna_ctrl1h_rep2_root.35200000074a0f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13774075
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	8418691	61.11982837323014	Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	190129	1.380339514631654	Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	72083	0.5233237077625902	Illumina RNA PCR Primer (97% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	49927	0.3624708011245764	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	45502	0.3303452318939747	Illumina RNA PCR Primer (97% over 46bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	27142	0.1970513446456477	No Hit
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	25849	0.18766414441623122	Illumina RNA PCR Primer (100% over 34bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	23921	0.17366683425202784	Illumina RNA PCR Primer (97% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	21970	0.15950254372798173	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	19728	0.14322558865114354	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	18117	0.13152970344651094	Illumina RNA PCR Primer (100% over 38bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	18061	0.13112314257037225	Illumina RNA PCR Primer (100% over 40bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	14182	0.10296154188212275	Illumina RNA PCR Primer (97% over 46bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	898010	44.62009	45
CGCCGTA	936450	43.26505	44
TCGCCGT	935915	43.23168	43
GGTCGCC	938355	43.197994	41
TGGTCGC	938825	43.15967	40
GTGGTCG	940110	43.112976	39
GTCGCCG	941505	43.056015	42
GGTGGTC	942835	42.95472	38
CGGTGGT	948675	42.667957	37
TCGGTGG	955935	42.354263	36
GCCGTAA	9255	42.349064	45
CTCGGTG	963605	42.00258	35
TCTCGGT	975455	41.505447	34
ACGATGT	3500	41.132263	7
ATCTCGG	988995	40.960625	33
CCGTATC	7815	40.82436	45
GATCTCG	996110	40.682594	32
TAACGCA	1660	40.657074	36
AGCCTGA	4515	40.629772	1
ACGAATC	5895	40.610954	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content