H72FYBCXY_l01n02_smallrna_ctrl1h_rep1_root.352000000749e4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n02_smallrna_ctrl1h_rep1_root.352000000749e4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19515275
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	10830372	55.49689666171756	Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	262674	1.3459917936078278	Illumina RNA PCR Primer (100% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	129535	0.6637621043003494	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	92991	0.47650366187512094	Illumina RNA PCR Primer (97% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	60397	0.30948577460476473	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	44064	0.22579236008716247	Illumina RNA PCR Primer (97% over 46bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	43585	0.2233378725126856	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	39865	0.2042758813288565	Illumina RNA PCR Primer (97% over 46bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	33969	0.17406365014072311	Illumina RNA PCR Primer (100% over 34bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	30440	0.1559803794719777	No Hit
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	26091	0.13369527203690443	Illumina RNA PCR Primer (100% over 38bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	22887	0.11727736350115485	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCATCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	20594	0.10552759312897206	Illumina RNA PCR Primer (100% over 46bp)
GATCGTGGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	20499	0.10504079496701942	Illumina RNA PCR Primer (97% over 46bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	20271	0.10387247937833313	Illumina RNA PCR Primer (100% over 34bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	19573	0.10029579393577594	Illumina RNA PCR Primer (100% over 35bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	1159915	44.519257	45
CGCCGTA	1213245	43.05794	44
TCGCCGT	1212775	43.029655	43
GGTCGCC	1216555	42.98056	41
TGGTCGC	1217130	42.941917	40
GTGGTCG	1219715	42.87334	39
GTCGCCG	1220220	42.844933	42
GGGAATG	15830	42.798332	2
GGTGGTC	1226025	42.61946	38
CGGTGGT	1238415	42.188988	37
GGAATAC	3885	42.033722	6
GCCGTAA	12650	41.798904	45
TCGGTGG	1252515	41.703846	36
CCGTATC	12760	41.5091	45
AGTAGTG	2705	41.37581	1
CTCGGTG	1267665	41.19376	35
GAATACT	3990	40.928516	7
GGGAATA	3970	40.917767	5
TCTCGGT	1287625	40.55921	34
ACTATAC	4095	40.537914	18

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content