FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l01n02_smallrna_ctrl025h_rep3_root.352000000749ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l01n02_smallrna_ctrl025h_rep3_root.352000000749ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308684
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT271421620.39432298490219Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT2803002.1061436277245744Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG968390.7276376837860152Illumina RNA PCR Primer (100% over 34bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT857450.6442785778067914Illumina RNA PCR Primer (100% over 35bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT850060.6387258124094013Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT758290.5697708353432991Illumina RNA PCR Primer (100% over 35bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT679770.5107717637596625No Hit
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA674460.5067818876757462No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA630230.473547948091637Illumina RNA PCR Primer (100% over 45bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG532520.4001297198130183Illumina RNA PCR Primer (100% over 33bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG499200.3750934352337166Illumina RNA PCR Primer (100% over 34bp)
GGCTGCGCCGACGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT482220.36233484843430047Illumina RNA PCR Primer (100% over 31bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT419960.3155533635031082Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG410360.3083400282101521Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG382900.2877068837159256Illumina RNA PCR Primer (100% over 37bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT377710.28380717432317126Illumina RNA PCR Primer (100% over 38bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC376420.2828378823931803Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG341910.25690744479318917Illumina RNA PCR Primer (100% over 36bp)
GCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG327510.24608744185375506Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG315430.23701066161011863Illumina RNA PCR Primer (100% over 36bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG315100.23676270320942325Illumina RNA PCR Primer (100% over 34bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG314250.23612402248035944No Hit
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA309510.23256243817946237Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC293630.22063037938236418Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC278310.2091190984773551No Hit
GCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC277240.20831511214782766Illumina RNA PCR Primer (100% over 32bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG272280.20458822224646706Illumina RNA PCR Primer (100% over 37bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA266250.200057346015579Illumina RNA PCR Primer (97% over 45bp)
GGCCGGCCGCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG261970.19684140069746942Illumina RNA PCR Primer (100% over 33bp)
CGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT259240.194790108473535Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA252090.18941767645846877No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG251430.18892175965707803Illumina RNA PCR Primer (100% over 34bp)
AGGGATGCAACACGAGGACTTCCCAGGAGGTCACCCATCCTAGTACTACTC241320.18132521592668366No Hit
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT231470.17392403336047352Illumina RNA PCR Primer (100% over 35bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC231080.17363099161419718Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG230690.17333794986792084Illumina RNA PCR Primer (100% over 36bp)
CCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT230600.17327032484954935Illumina RNA PCR Primer (100% over 31bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT229030.17209064397351384Illumina RNA PCR Primer (100% over 38bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG225820.16967868498493163Illumina RNA PCR Primer (100% over 34bp)
CGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT225490.16943072658423627Illumina RNA PCR Primer (100% over 31bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC216960.1630213776208076Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGTCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC191310.14374824738494055Illumina RNA PCR Primer (100% over 32bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG190230.14293674716448296No Hit
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT185120.13909714889916988Illumina RNA PCR Primer (100% over 35bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT177930.133694661320383Illumina RNA PCR Primer (100% over 38bp)
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG174650.13123010509528968Illumina RNA PCR Primer (100% over 36bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC173170.1301180492376256No Hit
GCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG166380.1250161172960452Illumina RNA PCR Primer (100% over 37bp)
AGGCCAGGAGCGCATCGCCGGCAGAAGGGTCGAGCAGGTCGGTGCTCGCCG164800.12382892252907952No Hit
GCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG157000.1179680876035527Illumina RNA PCR Primer (100% over 37bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA149390.11225001660569896Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG148200.11135586358500961Illumina RNA PCR Primer (100% over 37bp)
CCGGCTGCGCCGACGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA144200.10835030721294456Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG140700.10572044538738766Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG135840.10206869439532865Illumina RNA PCR Primer (100% over 37bp)
AGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT134490.1010543191197567Illumina DpnII expression Sequencing Primer (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2900500.044.46660645
GGGAATG330550.043.890362
TAACCTA46900.043.211511
CGCCGTA3037600.042.98574444
ATTACCT11950.042.7308410
GGTCGCC3062300.042.72850441
GATAACC48350.042.7111369
TCGCCGT3056500.042.6960643
ATAACCT48050.042.69577410
TGGTCGC3069200.042.59945340
ACTATAC88250.042.26183318
GTGGTCG3098350.042.19851339
CACTATA88500.042.09161817
GTCGCCG3118750.041.9399642
TATACTA89200.041.8404320
CGATAAC49450.041.8055888
GGAATAC88450.041.7329836
GGATGCA41100.041.708173
GGGATGC41300.041.622942
GAATACT88900.041.5985877
Produced by FastQC (version 0.11.2)