H72FYBCXY_l01n02_smallrna_ctrl025h_rep2_root.352000000749a1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n02_smallrna_ctrl025h_rep2_root.352000000749a1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14689319
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	5164570	35.158675497482214	Illumina RNA PCR Primer (100% over 46bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	116214	0.7911462743780021	Illumina RNA PCR Primer (100% over 45bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	108564	0.7390676177704358	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	72500	0.4935558959540602	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	67606	0.46023917106027856	Illumina RNA PCR Primer (100% over 34bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	67494	0.45947671229687365	Illumina RNA PCR Primer (100% over 35bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	65682	0.4471412187317874	Illumina RNA PCR Primer (100% over 34bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	62099	0.4227493459703612	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	54429	0.37053453601218683	Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	53614	0.36498628697491015	Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	51880	0.3531817914771951	Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	45364	0.30882302984910326	Illumina RNA PCR Primer (100% over 37bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	41808	0.28461496411099796	Illumina RNA PCR Primer (97% over 45bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	36918	0.2513254698873379	Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	36697	0.24982097536311929	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	35607	0.24240061775498237	No Hit
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	34367	0.23395911001728534	Illumina RNA PCR Primer (100% over 38bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	32826	0.2234684943529377	Illumina RNA PCR Primer (100% over 34bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	28799	0.19605401720801352	Illumina RNA PCR Primer (97% over 46bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG	27507	0.18725851075873565	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	26447	0.18004238317651078	Illumina RNA PCR Primer (100% over 37bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	25611	0.17435117312109566	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	25017	0.17030741860803758	Illumina RNA PCR Primer (100% over 33bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	23636	0.16090602974855403	Illumina RNA PCR Primer (100% over 37bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	21814	0.1485024595081637	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG	18701	0.12731019048602593	Illumina RNA PCR Primer (100% over 40bp)
TCGCGACGTCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	17961	0.12227251651352933	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT	17547	0.11945414215594338	Illumina RNA PCR Primer (100% over 31bp)
GGCCGGCCGCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	16998	0.11571673268175332	Illumina RNA PCR Primer (100% over 33bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	16804	0.11439604518085555	Illumina RNA PCR Primer (100% over 34bp)
CCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT	15089	0.10272089536621813	Illumina RNA PCR Primer (100% over 31bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	14940	0.1017065529041884	Illumina RNA PCR Primer (100% over 38bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	552570	44.52809	45
ATAACCT	3235	43.456406	10
TAACCTA	3160	43.346252	11
CGATAAC	3425	43.344986	8
CGCCGTA	577225	43.052395	44
TCGCCGT	577950	42.96748	43
GGTCGCC	579720	42.91616	41
TGGTCGC	580900	42.80535	40
ATTACCT	1110	42.757034	10
GATAACC	3370	42.651314	9
GTCGCCG	583795	42.617367	42
GTGGTCG	586845	42.394245	39
CATATGC	1705	42.215954	16
CACTATA	4745	42.047047	17
GGAATAC	4705	41.926155	6
ACGATGT	3935	41.899498	7
CCGTATC	5775	41.728054	45
GGGAATA	4710	41.654697	5
ACTATAC	4805	41.568535	18
GGGAATG	8760	41.43257	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content