FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l01n02_smallrna_ctrl025h_rep1_root.35200000074987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l01n02_smallrna_ctrl025h_rep1_root.35200000074987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16892212
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT356737921.11848347629073Illumina RNA PCR Primer (100% over 46bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG1601800.9482476303281062No Hit
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT1574680.9321928945717708Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG1425230.8437201711652683No Hit
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT986780.5841626898833616No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA955010.5653552062926986Illumina RNA PCR Primer (100% over 45bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG904330.5353532148424375Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG822870.4871298086952733Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT776890.4599101645184183Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC771740.456861422293303No Hit
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA700530.4147059011572907No Hit
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT677090.4008296841171541Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT645140.38191564254580745Illumina RNA PCR Primer (100% over 38bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG567030.33567539881692227Illumina RNA PCR Primer (100% over 34bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT562310.3328812117678845Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG541860.32077504118465955Illumina RNA PCR Primer (100% over 34bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC532450.31520442675003135Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT510460.3021865934431796Illumina RNA PCR Primer (100% over 38bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT501980.29716652857541687Illumina RNA PCR Primer (100% over 35bp)
TATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCGATCGTCGGACTGT451830.2674782911793908No Hit
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT440950.2610374532358462Illumina RNA PCR Primer (100% over 38bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC413590.24484064017193247Illumina RNA PCR Primer (100% over 39bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC408600.2418866161518693Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT358570.21226941740963234Illumina RNA PCR Primer (100% over 35bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT347370.2056391430559834Illumina RNA PCR Primer (97% over 46bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC314550.1862100712446659No Hit
CCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT307730.18217270775431899Illumina RNA PCR Primer (100% over 31bp)
GCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT277420.1642295277847567Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT267820.15854643548162906Illumina RNA PCR Primer (100% over 35bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG267080.15810836378326296Illumina RNA PCR Primer (100% over 33bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT261290.15468074873793913Illumina RNA PCR Primer (100% over 38bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA258220.15286334317850145Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA257200.1522595146212941No Hit
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT253410.15001587713912187Illumina RNA PCR Primer (97% over 46bp)
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG248430.1470677730068744Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG241900.14320208626318448Illumina RNA PCR Primer (100% over 37bp)
GCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG236390.13994022807670184Illumina RNA PCR Primer (100% over 34bp)
GCCCGGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG214420.12693423454548167Illumina RNA PCR Primer (100% over 37bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC210020.12432948390654819Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC206900.1224824789080317Illumina RNA PCR Primer (100% over 39bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCAGATCGTCGGA203570.1205111562653843No Hit
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG200250.11854575351055267Illumina RNA PCR Primer (100% over 34bp)
GCCCCGAGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG197350.11682898604398287Illumina RNA PCR Primer (100% over 36bp)
GGCCGGCCGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG197190.11673426783893075Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG186320.11029934978320187Illumina RNA PCR Primer (100% over 36bp)
GCCCCCGTCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC185670.10991455707517761Illumina RNA PCR Primer (100% over 32bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG183930.10888449659523572Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACGGGTTCGGGGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTG182510.1080438725253981Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG182500.10803795263758234Illumina RNA PCR Primer (100% over 36bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG181060.10718548879211319Illumina RNA PCR Primer (100% over 37bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG178760.10582391459448887Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCTCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG176050.10421962499641847Illumina RNA PCR Primer (100% over 37bp)
GCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG174150.10309484631142445Illumina RNA PCR Primer (100% over 37bp)
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT174040.10302972754545113Illumina RNA PCR Primer (100% over 35bp)
GGCCGGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT173310.10259757573490079Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA168940.10001058475941457Illumina DpnII expression Sequencing Primer (100% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3891650.044.2570545
TGGCCTC487200.043.608991
GGGAATG185500.043.1110042
CGATAAC51200.043.0977978
ATAACCT49150.043.01798210
CGCCGTA4106300.042.49209644
TCGCCGT4109300.042.4161743
GGTCGCC4127100.042.3635441
ACGATAA51600.042.2403537
TGGTCGC4133400.042.24034540
GGAATAC89700.042.0015646
GATAACC52700.041.9142889
TAACCTA49650.041.7667111
TATCTAG119100.041.71791
GTCGCCG4193200.041.6631342
GTGGTCG4196200.041.6249739
GGCCTCG517200.041.5584222
GGGAATA91200.041.3237765
GAATACT91050.041.305687
CACTATA91800.041.28674717
Produced by FastQC (version 0.11.2)