H72FYBCXY_l01n01_smallrna_heat_1h_rep1_root.35100000074a46.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n01_smallrna_heat_1h_rep1_root.35100000074a46.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11541825
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	5030554	43.58542951396335	RNA PCR Primer, Index 7 (100% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	71702	0.621236242968508	RNA PCR Primer, Index 7 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	64091	0.5552934652882018	RNA PCR Primer, Index 7 (98% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	56926	0.49321489452491263	RNA PCR Primer, Index 7 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	42206	0.3656787379812118	RNA PCR Primer, Index 7 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	41185	0.35683264994920644	RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAACATCTCGTATGCC	38873	0.33680115579641867	RNA PCR Primer, Index 7 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	37610	0.32585834562558347	RNA PCR Primer, Index 1 (100% over 30bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	35574	0.30821815440799005	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACATCATCTCGTATGCC	35373	0.30647666205301155	RNA PCR Primer, Index 7 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	34217	0.29646091497661764	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGCTCATCTCGTATGCC	33699	0.2919728899025934	RNA PCR Primer, Index 7 (98% over 51bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	28411	0.24615691192684	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAGCATCTCGTATGCC	27891	0.24165155857067666	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATGATCTCGTATGCC	26392	0.22866401110742887	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGATCATCTCGTATGCC	26348	0.22828278890036888	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGATCATCTCGTATGCC	24400	0.21140504209689542	RNA PCR Primer, Index 7 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	20926	0.18130581602129647	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAATCATCTCGTATGCC	19960	0.1729362557481161	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCTCGTATGCC	19292	0.16714860951366012	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGACCATCTCGTATGCC	18310	0.158640422983367	RNA PCR Primer, Index 7 (98% over 51bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC	17728	0.15359789288089187	RNA PCR Primer, Index 7 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATTATCTCGTATGCC	17355	0.15036616826195165	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGTTCATCTCGTATGCC	17121	0.14833875925167814	RNA PCR Primer, Index 7 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	16531	0.14322691602064666	RNA PCR Primer, Index 7 (98% over 51bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC	15651	0.13560247187944713	RNA PCR Primer, Index 7 (100% over 39bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC	15498	0.13427685829580677	RNA PCR Primer, Index 7 (100% over 39bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA	13741	0.11905396243661641	RNA PCR Primer, Index 7 (100% over 40bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGATCATCTCGTATGCC	13733	0.11898464930806002	RNA PCR Primer, Index 7 (98% over 51bp)
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATT	13410	0.11618613174259704	No Hit
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA	12897	0.11174142737392051	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGATCATCTCGTATGCC	12539	0.10863966487102343	RNA PCR Primer, Index 7 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	652210	44.069054	45
TCTCGTA	657635	43.72196	41
ATCTCGT	658375	43.715187	40
CGTATGC	657995	43.68163	44
CATCTCG	645385	43.64218	39
CTCGTAT	659895	43.63359	42
TCGTATG	659665	43.629704	43
TTATCTC	4630	43.585587	38
AGTTCTC	10830	43.570595	4
ACTAGAT	3440	43.490524	32
GTTCTCG	12690	43.446804	5
TCATCTC	625750	43.392654	38
GTCACTA	7390	43.16813	29
GTATAGT	2415	43.041023	10
CACTAGA	3880	42.965305	31
TGATCTC	5415	42.958935	38
TCACCAC	7565	42.912994	30
ATCATCT	621815	42.861526	37
GCATCTC	8015	42.80532	38
AGATTAT	2565	42.801983	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content