H72FYBCXY_l01n01_smallrna_ctrl1h_rep3_root.35100000074a2c.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n01_smallrna_ctrl1h_rep3_root.35100000074a2c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13678540
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	7777178	56.85678442289893	RNA PCR Primer, Index 3 (100% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	91118	0.6661383451742657	RNA PCR Primer, Index 3 (98% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	84100	0.6148316998744018	RNA PCR Primer, Index 3 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	66540	0.48645542579836737	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCGGCATCTCGTATGCC	65648	0.4799342619899492	RNA PCR Primer, Index 3 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	62597	0.45762924990532616	RNA PCR Primer, Index 3 (98% over 51bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	47172	0.34486136678329704	RNA PCR Primer, Index 3 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	46381	0.33907858587246886	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTATGCATCTCGTATGCC	45823	0.33499920313132836	RNA PCR Primer, Index 3 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	44484	0.32521014669694276	RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACGCATCTCGTATGCC	37484	0.27403509438872864	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGGCATCTCGTATGCC	37451	0.27379384057070416	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAAGCATCTCGTATGCC	33196	0.24268671948906828	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGGCATCTCGTATGCC	32984	0.24113684647630523	RNA PCR Primer, Index 3 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	32456	0.2372767853879142	RNA PCR Primer, Index 1 (100% over 30bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	32058	0.2343671181281043	RNA PCR Primer, Index 3 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	31311	0.22890600897464203	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGACATCTCGTATGCC	30625	0.22389085384843704	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGGCATCTCGTATGCC	26959	0.19708974788244946	RNA PCR Primer, Index 3 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	24459	0.17881294348665866	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGCCATCTCGTATGCC	24177	0.17675131995081347	RNA PCR Primer, Index 3 (98% over 51bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	23936	0.17498943600705924	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGGATCTCGTATGCC	23438	0.1713486965714177	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGTCATCTCGTATGCC	21806	0.1594175986618455	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAGGCATCTCGTATGCC	20483	0.149745513775593	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAGGCATCTCGTATGCC	17272	0.12627078620963933	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGTATCTCGTATGCC	16893	0.12350002266323745	RNA PCR Primer, Index 3 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16404	0.11992507972342077	RNA PCR Primer, Index 1 (100% over 33bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC	14565	0.1064806624098771	RNA PCR Primer, Index 3 (100% over 39bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTTCTCG	14805	44.23886	5
GTATGCC	964035	44.191452	45
TGCATCT	9950	44.113113	37
ATGCATC	8330	44.103664	36
AGTTCTC	13475	44.060555	4
TCTCGTA	969330	43.96782	41
ATCTCGT	970840	43.93728	40
CATCTCG	958465	43.93044	39
CTCGTAT	972485	43.89435	42
TCGTATG	972425	43.882713	43
CGTATGC	970955	43.87709	44
TATGCAT	6190	43.86823	35
TACGCAT	4940	43.856388	35
GCATCTC	941520	43.777958	38
CGGCATC	8995	43.644325	36
TCACTTC	12265	43.545364	30
ACGCATC	5740	43.54474	36
GGGATTC	8725	43.532967	2
TTACGCA	4580	43.472168	34
GATTCTC	10795	43.444542	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content