H72FYBCXY_l01n01_smallrna_ctrl1h_rep2_root.35100000074a02.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n01_smallrna_ctrl1h_rep2_root.35100000074a02.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13774075
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	7529694	54.665696244575415	RNA PCR Primer, Index 1 (100% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACCATCTCGTATGCC	92992	0.6751233748908729	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	91508	0.6643495116731977	RNA PCR Primer, Index 1 (98% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	79419	0.5765831825367584	RNA PCR Primer, Index 1 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	71376	0.5181908767013392	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCCCGATCTCGTATGCC	59259	0.4302212671268306	RNA PCR Primer, Index 1 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	58572	0.4252336363784864	RNA PCR Primer, Index 1 (98% over 51bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	47546	0.34518470387303685	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAACGATCTCGTATGCC	46817	0.3398921524675886	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCACGATCTCGTATGCC	46490	0.33751812735156445	RNA PCR Primer, Index 1 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	44758	0.32494378025384646	RNA PCR Primer, Index 1 (100% over 34bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	43239	0.3139158164885845	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAACACGATCTCGTATGCC	39342	0.28562353551871905	RNA PCR Primer, Index 1 (98% over 51bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	33581	0.2437985853859515	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAAGATCTCGTATGCC	31247	0.22685370886974263	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCTCGATCTCGTATGCC	30373	0.22050845519572096	RNA PCR Primer, Index 1 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	29811	0.2164283264030434	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCACGATCTCGTATGCC	26738	0.19411829832493288	RNA PCR Primer, Index 1 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	23781	0.1726504320616811	RNA PCR Primer, Index 1 (98% over 51bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG	22845	0.16585505741764872	RNA PCR Primer, Index 1 (100% over 39bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACATCACGATCTCGTATGCCG	20373	0.1479082987423838	RNA PCR Primer, Index 1 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATATCGTATGCC	18290	0.13278568615315364	RNA PCR Primer, Index 1 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17587	0.12768189515448405	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCGCGATCTCGTATGCC	17205	0.12490856917796657	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACATCACGATCTCGTATGCCG	16590	0.12044365955608634	TruSeq Adapter, Index 1 (96% over 33bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACATCACGATCTCGTATGCCG	15650	0.11361924484947264	Illumina PCR Primer Index 1 (96% over 32bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	15613	0.11335062427059531	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGACGATCTCGTATGCC	14153	0.10275100142840808	RNA PCR Primer, Index 1 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CCATCTC	18510	44.33777	38
AGTTCTC	13880	44.172245	4
ACCATCT	14745	44.170345	37
GTTCTCG	15490	44.08718	5
CTATCTC	3775	44.04056	38
GTATGCC	943475	43.956417	45
TCTCGTA	942220	43.900536	41
ATCTCGT	943450	43.86945	40
GATCTCG	915585	43.868477	39
GGGATTC	9250	43.814926	2
GGTATTC	4235	43.809177	2
CTCGTAT	947015	43.784912	42
CGTATGC	947300	43.772694	44
TCGTATG	949200	43.757122	43
CGATCTC	907055	43.756695	38
TCACAGC	9740	43.746655	30
TCACCAT	11350	43.725685	35
TGGAGTT	11375	43.711143	1
GATTCTC	11430	43.69247	4
CACATAA	7175	43.677185	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content