H72FYBCXY_l01n01_smallrna_ctrl1h_rep1_root.351000000749e7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n01_smallrna_ctrl1h_rep1_root.351000000749e7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19515275
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	9741328	49.91642700397509	RNA PCR Primer, Index 11 (100% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	114811	0.5883135133888711	RNA PCR Primer, Index 11 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	104718	0.5365950518247885	RNA PCR Primer, Index 1 (100% over 30bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	102089	0.5231235532166469	RNA PCR Primer, Index 11 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	90725	0.46489224466475615	RNA PCR Primer, Index 11 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	83436	0.4275420151650438	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCTACATCTCGTATGCC	76695	0.39299984243112124	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTACATCTCGTATGCC	72001	0.36894688903948314	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACTACATCTCGTATGCC	61715	0.3162394585779601	RNA PCR Primer, Index 11 (98% over 51bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	59486	0.30481763644119797	RNA PCR Primer, Index 11 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	54500	0.27926841922545287	RNA PCR Primer, Index 6 (100% over 34bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	53671	0.27502046473851893	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCTACATCTCGTATGCC	48976	0.2509623871557024	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGATACATCTCGTATGCC	46046	0.23594850700284775	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAGATCTCGTATGCC	44569	0.22838007663227908	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCCACATCTCGTATGCC	43717	0.2240142657482408	RNA PCR Primer, Index 11 (98% over 51bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	39764	0.2037583380198332	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTCCATCTCGTATGCC	39631	0.2030768205930995	RNA PCR Primer, Index 11 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	39324	0.20150369390131576	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTACATCTCGTATGCC	39099	0.2003507508861648	RNA PCR Primer, Index 11 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	34667	0.1776403355832803	RNA PCR Primer, Index 11 (98% over 51bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	31192	0.1598337712381711	RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC	29520	0.15126612358780492	RNA PCR Primer, Index 11 (100% over 39bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	28583	0.14646475645359855	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	27809	0.14249863248147926	RNA PCR Primer, Index 11 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGCTACATCTCGTATGCCG	24763	0.12689034615192457	TruSeq Adapter, Index 11 (96% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAATCTCGTATGCCG	22961	0.11765655364836007	RNA PCR Primer, Index 11 (97% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTGCATCTCGTATGCC	22720	0.11642162357435393	RNA PCR Primer, Index 11 (98% over 51bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA	22242	0.11397226019105547	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCAACATCTCGTATGCC	21610	0.11073377136627591	RNA PCR Primer, Index 11 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTTCTCG	20130	43.967644	5
GTATGCC	1222405	43.919765	45
AGTTCTC	18125	43.91222	4
TCTACAT	11320	43.782024	35
CATCTCG	1211760	43.725063	39
TCTCGTA	1228825	43.720028	41
ACTACAT	8470	43.7194	35
ATCTCGT	1230755	43.6854	40
CACACGA	3330	43.654774	1
CTCGTAT	1232495	43.63986	42
CGTATGC	1230000	43.63839	44
TCGTATG	1232405	43.62004	43
TGCATCT	7095	43.535736	37
ACATCTC	1199805	43.505714	38
GGGATTC	10765	43.31517	2
CATTCCT	5475	43.309277	16
TGGAGTT	14880	43.25851	1
GGTATTC	5220	43.218647	2
ACGACTA	7965	43.21467	32
CTATATC	3260	43.200077	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content