FastQCFastQC Report
Fri 23 Dec 2016
H72FYBCXY_l01n01_smallrna_ctrl025h_rep3_root.351000000749cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72FYBCXY_l01n01_smallrna_ctrl025h_rep3_root.351000000749cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308684
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC240935818.10365322371468RNA PCR Primer, Index 10 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2242821.6852304855987261RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT859070.6454958281374777RNA PCR Primer, Index 10 (100% over 39bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA785490.5902086186733414RNA PCR Primer, Index 10 (100% over 40bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT760990.571799585894443RNA PCR Primer, Index 3 (100% over 34bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA575920.43274000644992394RNA PCR Primer, Index 10 (100% over 40bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG507090.38102189517761487No Hit
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA491590.3693753642358628No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTCATCTCGTATGCC474140.35626362456272914RNA PCR Primer, Index 10 (98% over 51bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT467680.35140965102184407RNA PCR Primer, Index 10 (100% over 38bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT371570.27919364529205143RNA PCR Primer, Index 10 (100% over 39bp)
GCCGTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG358290.2692151981367955RNA PCR Primer, Index 7 (97% over 36bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT306970.23065390988320106No Hit
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC302160.22703972834579286RNA PCR Primer, Index 10 (98% over 51bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT302120.2270096727820722RNA PCR Primer, Index 1 (100% over 30bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA294040.22093844891050082RNA PCR Primer, Index 1 (100% over 32bp)
GCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC293510.22054021269120222RNA PCR Primer, Index 10 (100% over 42bp)
GCCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT288390.21669310053495897RNA PCR Primer, Index 10 (100% over 39bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA255050.1916417881737969RNA PCR Primer, Index 7 (97% over 35bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT250700.18837324561917615RNA PCR Primer, Index 10 (100% over 43bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC250420.18816285667313162RNA PCR Primer, Index 10 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC242870.18248986902085887RNA PCR Primer, Index 1 (100% over 33bp)
GCCCCCGACGTCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA242120.18192632720109667RNA PCR Primer, Index 7 (97% over 35bp)
GGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT237030.17810175671764392RNA PCR Primer, Index 10 (100% over 41bp)
CCGACCTGCTCGACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT233450.17541178376464572RNA PCR Primer, Index 3 (100% over 34bp)
GCCCCCGACGTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC231390.1738639222330322RNA PCR Primer, Index 10 (100% over 37bp)
GGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT225140.1691677404016806RNA PCR Primer, Index 10 (100% over 41bp)
GCCGCGGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT224690.16882961530982327RNA PCR Primer, Index 10 (100% over 38bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC218500.16417851682405263RNA PCR Primer, Index 10 (98% over 51bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT213850.16068455754152702RNA PCR Primer, Index 10 (100% over 41bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT210660.15828762633480514RNA PCR Primer, Index 10 (100% over 39bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT207690.15605600072854686RNA PCR Primer, Index 10 (100% over 39bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA205210.15419255577786656No Hit
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC203980.15326834719345653RNA PCR Primer, Index 10 (100% over 42bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA203400.15283254151950712RNA PCR Primer, Index 7 (97% over 35bp)
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA198750.14933858223698152RNA PCR Primer, Index 10 (100% over 40bp)
GCCCCCGACGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG191990.1442591919681916RNA PCR Primer, Index 7 (97% over 36bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC185360.1392774822814938RNA PCR Primer, Index 10 (98% over 51bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC177080.13305598059131918No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCGTATCTCGTATGCC169480.1273454234843956RNA PCR Primer, Index 10 (98% over 51bp)
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT168240.12641370100905544RNA PCR Primer, Index 10 (100% over 43bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT166510.1251137978781373RNA PCR Primer, Index 10 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCATATCTCGTATGCC164160.1233480335095491RNA PCR Primer, Index 10 (98% over 51bp)
GGGGGACGGACCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG163400.12277697779885675RNA PCR Primer, Index 7 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA160720.12076325502957316RNA PCR Primer, Index 7 (97% over 35bp)
GCGGCGACGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC158100.11879461560587057RNA PCR Primer, Index 10 (100% over 37bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC157410.11827615713168936RNA PCR Primer, Index 10 (98% over 51bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC157410.11827615713168936No Hit
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATT156740.11777272643936847No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCCTATCTCGTATGCC152320.1144515866482366RNA PCR Primer, Index 10 (98% over 51bp)
GGGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC149450.11229509995127993RNA PCR Primer, Index 10 (100% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTGATCTCGTATGCC148050.11124315522105717RNA PCR Primer, Index 10 (98% over 51bp)
CCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA141660.10644177891668329RNA PCR Primer, Index 10 (100% over 40bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC139470.10479623680297767RNA PCR Primer, Index 10 (98% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCC336950.044.3676214
GTATGCC3047850.043.9831745
CGTATGC3074800.043.6024644
CACACGA67550.043.595171
TCGTATG3092600.043.43515443
CTCGTAT3098350.043.37996342
GTATAGT90000.043.32286510
TCTCGTA3098650.043.30969241
ATCTCGT3109350.043.18959440
TAGTATA91400.043.176218
TATAGTG90600.043.03595711
CGTTGTA100250.043.02433
TGTAGTA89600.042.7399336
AGTATAG91700.042.6423879
TATTCCC89700.042.5623923
GTAGTAT92100.042.4816177
TCATCTC82600.042.4625438
GTGAGTA89950.042.2940318
GAGTATT89600.042.2332620
AGTATTC90300.042.2297621
Produced by FastQC (version 0.11.2)