H72FYBCXY_l01n01_smallrna_ctrl025h_rep2_root.351000000749a4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n01_smallrna_ctrl025h_rep2_root.351000000749a4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14689319
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	4633810	31.545437879046673	RNA PCR Primer, Index 9 (100% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	99288	0.6759196937584376	RNA PCR Primer, Index 6 (100% over 34bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	61044	0.4155672567257883	RNA PCR Primer, Index 9 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	59064	0.4020880750155947	RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	58377	0.3974112074222093	RNA PCR Primer, Index 9 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACCAGATCTCGTATGCC	55741	0.37946619581207275	RNA PCR Primer, Index 9 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCACATCTCGTATGCC	54399	0.37033030598627475	RNA PCR Primer, Index 9 (98% over 51bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA	51528	0.350785492506494	RNA PCR Primer, Index 9 (100% over 40bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA	50100	0.34106414327308165	RNA PCR Primer, Index 9 (100% over 40bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	47474	0.32318720833824904	RNA PCR Primer, Index 7 (97% over 35bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	45820	0.31192732624296604	RNA PCR Primer, Index 9 (98% over 51bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	45422	0.3092178745658665	RNA PCR Primer, Index 9 (100% over 39bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	42482	0.2892033320264881	RNA PCR Primer, Index 9 (98% over 51bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT	35537	0.24192408102785432	RNA PCR Primer, Index 9 (100% over 43bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT	34563	0.2352934128532439	RNA PCR Primer, Index 9 (100% over 41bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	33532	0.22827470762940066	RNA PCR Primer, Index 9 (98% over 51bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC	33459	0.2277777478996814	RNA PCR Primer, Index 9 (100% over 42bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	31298	0.21306637836648518	RNA PCR Primer, Index 9 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	29263	0.1992127749421195	RNA PCR Primer, Index 1 (100% over 33bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	27002	0.18382063865588322	RNA PCR Primer, Index 9 (98% over 51bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	26989	0.18373213897798804	RNA PCR Primer, Index 9 (100% over 39bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA	26326	0.17921865540533227	No Hit
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT	25316	0.17234291119962744	RNA PCR Primer, Index 9 (100% over 43bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	24347	0.16574628136266903	RNA PCR Primer, Index 9 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAACAGATCTCGTATGCC	23712	0.1614234124808645	RNA PCR Primer, Index 9 (98% over 51bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	23215	0.1580400017182553	RNA PCR Primer, Index 7 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCCGATCTCGTATGCC	22209	0.15119148818267203	RNA PCR Primer, Index 9 (98% over 51bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA	21838	0.14866584352889334	RNA PCR Primer, Index 9 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGCAGATCTCGTATGCC	21522	0.1465146205892867	RNA PCR Primer, Index 9 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTCAGATCTCGTATGCC	20421	0.13901937863831534	RNA PCR Primer, Index 9 (98% over 51bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	20277	0.13803907451393765	RNA PCR Primer, Index 9 (98% over 51bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	19781	0.13466247141885884	RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTCAGATCTCGTATGCC	19174	0.13053021722790553	RNA PCR Primer, Index 9 (98% over 51bp)
GGCGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC	18841	0.12826326394028206	RNA PCR Primer, Index 9 (100% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATAAGATCTCGTATGCC	18207	0.12394720272600793	RNA PCR Primer, Index 9 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	17213	0.11718038120078951	RNA PCR Primer, Index 9 (98% over 51bp)
CCGGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC	15134	0.10302724040508618	RNA PCR Primer, Index 9 (100% over 42bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	15068	0.10257793434807971	RNA PCR Primer, Index 7 (97% over 35bp)
GCCCCCGACGTCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	15024	0.10227839697674208	RNA PCR Primer, Index 7 (97% over 35bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	581950	44.374504	45
CGTATGC	585165	44.12844	44
TCTCGTA	584755	44.080124	41
CTCGTAT	586205	44.080082	42
TCGTATG	587240	44.038406	43
ATCTCGT	586535	43.966293	40
CACACGA	5485	43.752045	1
GATCTCG	572465	43.6502	39
CACATCT	11120	43.33603	37
CGTTGTA	10605	43.03166	3
TAGTATA	4885	43.015858	8
TATAGTG	4805	42.982883	11
GTATAGT	4790	42.97658	10
CATTCCC	8820	42.954285	14
AGATCTC	575990	42.69587	38
TCCGTTG	7330	42.515137	1
TAGTCTA	9945	42.507767	8
GATCACA	6455	42.485615	34
GTTGTAG	11415	42.384346	4
CATCTCG	14900	42.2772	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content