H72FYBCXY_l01n01_smallrna_ctrl025h_rep1_root.3510000007498a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H72FYBCXY_l01n01_smallrna_ctrl025h_rep1_root.3510000007498a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16892212
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	3225732	19.095971563700477	RNA PCR Primer, Index 2 (100% over 51bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT	139399	0.8252264416288405	No Hit
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC	132785	0.7860723036154175	No Hit
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	128593	0.7612561338917603	RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	75057	0.44432901978734346	RNA PCR Primer, Index 2 (100% over 39bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	74502	0.4410434820495978	RNA PCR Primer, Index 2 (100% over 34bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG	74008	0.4381190574686134	No Hit
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	63758	0.3774402073570945	RNA PCR Primer, Index 2 (100% over 40bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	62011	0.3670981633429654	RNA PCR Primer, Index 2 (100% over 39bp)
CCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATT	56740	0.3358944346661053	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGCATCTCGTATGCC	56096	0.3320820269127572	RNA PCR Primer, Index 2 (98% over 51bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA	50613	0.29962328201895644	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGCC	46214	0.2735816955174373	RNA PCR Primer, Index 2 (98% over 51bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	45263	0.2679518822046515	RNA PCR Primer, Index 2 (100% over 39bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	44100	0.26106705267492497	RNA PCR Primer, Index 2 (100% over 43bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	42406	0.25103876271503106	RNA PCR Primer, Index 2 (100% over 39bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	41156	0.24363890294533364	RNA PCR Primer, Index 2 (98% over 51bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	40793	0.2414899836682135	RNA PCR Primer, Index 1 (100% over 30bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	39427	0.23340341691188818	RNA PCR Primer, Index 2 (100% over 40bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	36785	0.21776307330265568	RNA PCR Primer, Index 2 (100% over 35bp)
GCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGGAATTCTCGGGT	34736	0.20563322316816765	No Hit
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	34718	0.20552666518748403	RNA PCR Primer, Index 2 (98% over 51bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	34666	0.2052188310210646	RNA PCR Primer, Index 2 (100% over 43bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	32997	0.1953385382565646	RNA PCR Primer, Index 2 (98% over 51bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	31330	0.18547008526769615	RNA PCR Primer, Index 2 (100% over 35bp)
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	30321	0.17949691846159638	RNA PCR Primer, Index 2 (100% over 40bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	28846	0.17076508393335343	RNA PCR Primer, Index 2 (100% over 43bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	26029	0.1540887599563633	RNA PCR Primer, Index 2 (100% over 44bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG	24690	0.14616203017106344	RNA PCR Primer, Index 2 (100% over 38bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	24396	0.14442158315323061	RNA PCR Primer, Index 2 (98% over 51bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA	23397	0.13850761522528843	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAAGTATCTCGTATGCC	22593	0.13374802542141906	RNA PCR Primer, Index 2 (98% over 51bp)
GGGGGACGGACCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA	22242	0.13167014479808803	RNA PCR Primer, Index 2 (100% over 36bp)
GCCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	22110	0.13088871960640797	RNA PCR Primer, Index 2 (100% over 39bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21907	0.1296869823798091	RNA PCR Primer, Index 1 (100% over 33bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	21280	0.1259752127193289	RNA PCR Primer, Index 2 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGACGTATCTCGTATGCC	20809	0.1231869455581069	RNA PCR Primer, Index 2 (98% over 51bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	20405	0.12079531088054068	RNA PCR Primer, Index 2 (100% over 35bp)
CCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	19930	0.11798336416805567	RNA PCR Primer, Index 2 (100% over 40bp)
CCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	19790	0.11715457987384957	RNA PCR Primer, Index 2 (100% over 43bp)
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	19699	0.11661587008261558	RNA PCR Primer, Index 2 (100% over 43bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG	19646	0.11630211602838042	RNA PCR Primer, Index 2 (98% over 51bp)
GGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT	19513	0.11551477094888461	RNA PCR Primer, Index 2 (100% over 41bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC	18895	0.1118562802787462	RNA PCR Primer, Index 2 (100% over 42bp)
TGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCTC	18585	0.11002111505586125	No Hit
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	18309	0.10838722601871206	RNA PCR Primer, Index 2 (98% over 51bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC	18046	0.10683029552316771	No Hit
TCCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC	17720	0.10490041209523064	RNA PCR Primer, Index 2 (100% over 42bp)
CCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT	17706	0.10481753366581002	RNA PCR Primer, Index 2 (100% over 41bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAGGTATCTCGTATGCC	17587	0.10411306701573482	RNA PCR Primer, Index 2 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	414265	43.83875	45
CATTCCC	18995	43.573757	14
CGTATGC	417610	43.48928	44
CACACGA	8170	43.48915	1
TCTCGTA	418355	43.43012	41
CTCGTAT	419065	43.42633	42
CATTCCT	7025	43.425804	16
TCGTATG	419485	43.365685	43
TGCATCT	9515	43.31654	37
ATCTCGT	420645	43.218693	40
GCATCTC	11795	43.126053	38
GTATAGT	9325	42.8986	10
TAGTATA	9485	42.64937	8
CATCTCG	13605	42.63035	39
AGTATAG	9390	42.62561	9
TATAGTG	9410	42.60674	11
ATGCATC	8115	42.5279	36
GTAGTAT	9505	42.157227	7
AGTATTC	9275	42.108555	21
GAGTATT	9245	42.099186	20

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content