FastQCFastQC Report
Fri 7 Feb 2020
H72CGBGXF_n02_Salt_NSF_Plate_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72CGBGXF_n02_Salt_NSF_Plate_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370306669
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44360921.1979508800042702No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT9937330.2683540652085853No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG5415450.1462423027547473No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5095700.1376075676347055No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG4077010.11009820619784733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA871650.021.5709111
GTATCAA3058650.020.263131
GGACCAT2167550.019.3283446
GTAGATC1743200.019.15248543
GTTATAC3258950.019.0414893
TAGATCT1744100.018.7052844
ATACCGG3385300.018.4233386
GGGGGTA3231600.018.37777321
TCAGGGG3267650.018.16726718
GGGGTAT3284950.017.8323722
TACCGGT3493000.017.8036547
TATACCG3587750.017.7684425
TGGACCA2534250.017.75985
GGTATTC3279600.017.71779824
TGTTATA3602650.017.6093042
AGGGGGT3378650.017.54554420
GGCTTCA3385050.017.54378514
GGGTATT3342650.017.5374523
CAGGGGG3397150.017.49349619
TTATACC3616950.017.126424
Produced by FastQC (version 0.11.5)