FastQCFastQC Report
Fri 7 Feb 2020
H72CGBGXF_n01_Salt_NSF_Plate_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH72CGBGXF_n01_Salt_NSF_Plate_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370306669
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG69181271.868215611315388No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCTT4832000.013.862052
TACACCT4836300.013.8610621
CCTCTCT3569450.013.85537054
CGCATCC4459800.013.8420685
TTCCACC3196250.013.841252
CCTCAGT5335150.013.8406466
CCACACT3707450.013.8300052
TCAACCG3401700.013.8269591
TCGACTT3940900.013.8243346
TCGCATC4467650.013.8167014
CCTCTGA3854500.013.8137984
GTCCTCT3864300.013.8132022
TCCTTAC3194950.013.8085594
AACTACC3056150.013.8055676
AGAGACT5918100.013.8035365
CTCCTAC2385000.013.8020824
AACCGAT3419850.013.7955693
CGCATCT3600900.013.7951165
TCGACCA2993600.013.7937442
AGCGAAT5679100.013.7924056
Produced by FastQC (version 0.11.5)