FastQCFastQC Report
Tue 11 Feb 2020
H725TBGXF_n02_Salt_NSF_Plate_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH725TBGXF_n02_Salt_NSF_Plate_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448267234
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25833510.5762970844306681No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT10256530.22880391922644963No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG5776470.128862195625032No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5051030.11267899183548177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA980200.020.0437491
GTATCAA3445650.019.2149241
GTTATAC3735750.018.9036183
GGACCAT2276800.018.890186
ATACCGG3813300.018.4066526
TATACCG3990600.018.0689965
GGGGGTA3759900.017.78295121
TCAGGGG3768550.017.73945618
TACCGGT3947000.017.6209587
TGTTATA4195250.017.2744582
TGGACCA2699200.017.2680155
GGGGTAT3816200.017.24381322
TTATACC4101800.017.1967144
GGTATTC3797150.017.16464624
GGCTTCA3891000.017.16321614
CAGGGGG3953950.016.98409319
GGGTATT3893400.016.95127523
AGGGGGT3957050.016.86096220
TGGCATA3779850.016.57468836
GTAGATC1642850.016.49882943
Produced by FastQC (version 0.11.5)