FastQCFastQC Report
Tue 11 Feb 2020
H725TBGXF_n01_Salt_NSF_Plate_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH725TBGXF_n01_Salt_NSF_Plate_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448267234
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG51255641.1434170537657453No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC6411900.013.8981024
TTCCACC4369900.013.8706912
CCTCAGT7938100.013.8636736
TCCTTAC5698400.013.8586124
TCTCCTA6452450.013.8455843
CCTCTCT4407450.013.8447824
TCCTACG6424050.013.8357465
CCTACGT6408300.013.825976
TCGACTT4660150.013.8159466
CCTTACG5705550.013.8115515
AATCCTT5677150.013.8109412
CGCATCC4923500.013.8095435
TGCCTCA7994600.013.8094554
GCCTCAG7997850.013.7996415
TCAGAAC4338800.013.7944212
TCGACCA3536900.013.7935392
AGGACAC5483400.013.7926975
GTGCCTC8016500.013.7908533
AACTACC4338850.013.7904026
TCGCATC4934550.013.7838714
Produced by FastQC (version 0.11.5)