FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_input_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_input_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36254675
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37000.032.25668348
ATGCCGT38500.031.000147
TATGCCG38800.030.85027146
GTATGCC40150.030.07402645
CGTATGC39950.030.04953644
CACGGCT49400.024.72608431
ACGTCTG64700.018.60848415
GCTACAT70350.017.56154335
CATCTCG71650.017.14630339
GCACACG70900.017.12974211
CTACATC77100.016.25180636
ACACGTC77100.015.97940713
CACACGT78550.015.72884212
CGTCTGA76550.015.68213516
TCTCGTA80950.015.21978141
ATCTCGT89150.013.78049340
CTGAACT103650.012.02081619
GAGCACA109900.011.433829
TCTGAAC108000.011.24495718
AGCACAC112800.011.1396810
Produced by FastQC (version 0.11.2)