FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_input_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_input_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27792255
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC816270.29370412728294265TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG110800.054.9320846
CGTATGC112300.054.166744
ATGCCGT112250.054.1290547
GTATGCC114600.053.14056845
TGCCGTC114800.052.7740848
ACGTCTG134550.045.4174615
CGATCAG133400.045.28328733
GCACACG140850.043.3616311
CGTCTGA147000.041.5705816
ACACGTC149400.041.04445313
CACACGT150400.040.88760812
TCTCGTA148800.040.41224741
AGTCACG154450.039.54202728
GCCGTCT157250.038.9950749
GTCACGA156750.038.8951229
ATCTCGT161550.037.2442640
CTCGTAT168150.036.2819842
TCGTATG171750.036.11112643
CTGAACT173650.035.3920619
TCTGAAC177850.034.5954318
Produced by FastQC (version 0.11.2)