FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_input_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_input_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42763115
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC71000.045.30248648
TATGCCG80900.044.47454546
ATGCCGT75400.044.0055547
GTATGCC84950.043.83692645
CGTATGC82150.043.03032744
CACTAGC135000.040.41732431
ACGTCTG135700.040.3896115
GCACACG142950.038.21969211
GTCACTA145500.037.5969229
CGTCTGA151750.036.23269716
ACACGTC151300.036.22632613
CACACGT152550.035.88322412
AGTCACT165600.033.28698328
ACTAGCT165600.033.20252232
GCTTATC163700.033.0548236
CTGAACT185350.029.9667119
TCTGAAC187550.029.4843618
GAGCACA187600.029.3490669
TAGCTTA187750.029.2854934
TCTCGTA127900.028.24209241
Produced by FastQC (version 0.11.2)