FastQCFastQC Report
Mon 2 May 2016
H7237BGXY_n01_tga1_rep3_input_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_input_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42675612
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60150.031.71210746
GCACACG105050.030.61898611
ACGTCTG107200.030.29756515
GTATGCC68800.029.04743845
CGTATGC67150.028.82290544
TGCCGTC57800.027.91510848
ATGCCGT61500.027.7154347
GTCACAC118100.027.5904929
CACACGT120400.026.94762612
ACACGTC123750.026.35982513
CGTCTGA130100.025.07209416
AGCACAC161850.020.41524310
GAGCACA165100.019.9075059
CTGAACT175350.019.02134319
CACACTT175750.018.9186231
ACTCCAG183850.018.25586523
TCACACT184000.018.0702830
AGAGCAC183600.018.0348388
TCTGAAC185350.017.99493418
CTCCAGT191600.017.49933424
Produced by FastQC (version 0.11.2)