FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_input_180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_input_180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33007879
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28750.040.90403746
TGCCGTC28950.040.25900748
ATGCCGT28950.040.13847447
GTATGCC30950.038.22236345
CGTATGC30200.038.1284544
AGTCACC42900.028.06450328
ACGTCTG48150.024.71379915
GCACACG52250.022.70827111
CACACGT54600.022.11545212
CGTCTGA54300.021.97904416
ACACGTC55050.021.93470613
TCTCGTA51400.021.45003541
ATCTCGT51850.021.3311540
AATCTCG54150.020.5544239
CTGAACT65200.018.46558619
TGTAATC61100.018.38786136
TCTGAAC68850.017.8424618
AGCACAC73550.016.27567910
GAGCACA74900.016.0760339
GCTTGAA75950.015.29921659
Produced by FastQC (version 0.11.2)