FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_input_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_input_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40611453
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC610740.1503861484591551TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG102100.046.99810446
ATGCCGT99950.046.5736447
CGTATGC103950.046.2284544
TGCCGTC101250.044.9726848
GTATGCC109000.044.7932545
GCACACG148550.039.98287611
ACGTCTG149650.039.57073215
AGTCACC161300.036.71275328
CACACGT163950.036.39726312
ACACGTC165250.035.96307813
CGTCTGA171100.034.73270816
CATCTCG162450.030.05603239
TCTCGTA156150.029.96897741
CCAGATC199900.029.71122733
GAGCACA203750.029.4280879
CACCAGA206200.029.00714131
AGCACAC206200.028.97612210
CTGAACT206550.028.87333719
GCCGTCT155950.028.05376249
ACTCCAG215500.027.69005623
Produced by FastQC (version 0.11.2)