FastQCFastQC Report
Fri 29 Apr 2016
H7237BGXY_n01_tga1_rep3_chip_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7237BGXY_n01_tga1_rep3_chip_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34182246
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1814490.5308281966024117TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC415680.12160698861040319TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG262850.062.7025746
CGTATGC265650.062.26511444
GTATGCC269400.061.85291345
ATGCCGT263800.061.6679947
TGCCGTC262750.061.39420748
CACAGTG310750.057.40575833
GTCACAC322600.055.4816829
TCTCGTA244800.053.94590841
CAGTGAT329100.053.84294535
ACAGTGA336200.053.04963334
ATCTCGT259300.051.03642740
GCCGTCT320650.049.67527449
TGCTTGA339700.047.25104558
ACGTCTG386850.046.60167715
CGTCTGA387650.046.57813616
GCTTGAA347800.046.46209759
TCGTATG352500.046.36931643
CCGTCTT350000.046.32889650
ACACAGT390250.046.0431432
CTGAACT393600.045.8034919
Produced by FastQC (version 0.11.2)